APC Anti-CD18 antibody [MEM-48], prediluted (ab40914)
Key features and details
- APC Mouse monoclonal [MEM-48] to CD18, prediluted
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Overview
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Product name
APC Anti-CD18 antibody [MEM-48], prediluted
See all CD18 primary antibodies -
Description
APC Mouse monoclonal [MEM-48] to CD18, prediluted -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Species reactivity
Reacts with: Human -
Immunogen
Leucocytes of a patient suffering from a LGL-type leukaemia.
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Epitope
This antibody recognizes an epitope including amino acids 534-546 in the cysteine-rich repeat 3 of the CD18 antigen. -
General notes
The conjugate was purified by size-exclusion chromatography and adjusted for direct use.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
MEM-48 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. -
Involvement in disease
Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions. -
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
Post-translational
modificationsBoth Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3689 Human
- Omim: 600065 Human
- SwissProt: P05107 Human
- Unigene: 375957 Human
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Alternative names
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
see all
References (2)
ab40914 has been referenced in 2 publications.
- Lu C et al. Epitope mapping of antibodies to the C-terminal region of the integrin beta 2 subunit reveals regions that become exposed upon receptor activation. J Immunol 166:5629-37 (2001). PubMed: 11313403
- Bazil V et al. Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein. Folia Biol (Praha) 36:41-50 (1990). PubMed: 1971601
