APC Anti-CD19 antibody [LT19] (ab18224)
Key features and details
- APC Mouse monoclonal [LT19] to CD19
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Overview
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Product name
APC Anti-CD19 antibody [LT19]
See all CD19 primary antibodies -
Description
APC Mouse monoclonal [LT19] to CD19 -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD19. B lymphoblast cell line Daudi
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Positive control
- Human blood cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified by size exclusion chromatography. -
Clonality
Monoclonal -
Clone number
LT19 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Application notes
Flow Cyt: Use 20µl for 100µl human blood cells.
IP: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. -
Involvement in disease
Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 930 Human
- Omim: 107265 Human
- SwissProt: P15391 Human
- Unigene: 652262 Human
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Alternative names
- Antibody deficiency due to defect in CD19 antibody
- Antibody deficiency due to defect in CD19, included antibody
- AW495831 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab18224 has been referenced in 3 publications.
- Ding F et al. microRNA-375 released from extracellular vesicles of bone marrow mesenchymal stem cells exerts anti-oncogenic effects against cervical cancer. Stem Cell Res Ther 11:455 (2020). PubMed: 33109266
- Liu L et al. Pfit is a structurally novel Crohn's disease-associated superantigen. PLoS Pathog 9:e1003837 (2013). PubMed: 24385909
- Trepel M et al. Phenotypic detection of clonotypic B cells in multiple myeloma by specific immunoglobulin ligands reveals their rarity in multiple myeloma. PLoS One 7:e31998 (2012). Flow Cyt ; Human . PubMed: 22384124