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  1. Link

    apc-cd19-antibody-lt19-ab18224.pdf

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Immunology Adaptive Immunity B Cells CD
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APC Anti-CD19 antibody [LT19] (ab18224)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (2)

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Key features and details

  • APC Mouse monoclonal [LT19] to CD19
  • Reacts with: Human
  • Conjugation: APC. Ex: 645nm, Em: 660nm
  • Isotype: IgG1

Overview

  • Product name

    APC Anti-CD19 antibody [LT19]
    See all CD19 primary antibodies
  • Description

    APC Mouse monoclonal [LT19] to CD19
  • Host species

    Mouse
  • Conjugation

    APC. Ex: 645nm, Em: 660nm
  • Species reactivity

    Reacts with: Human
  • Immunogen

    B lymphoblast cell line Daudi.

  • Positive control

    • Human blood cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: PBS, BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified by size exclusion chromatography.
  • Clonality

    Monoclonal
  • Clone number

    LT19
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage

Associated products

  • Alternative Versions

    • PE Anti-CD19 antibody [LT19], prediluted (ab18229)
    • APC/Cy7® Anti-CD19 antibody [LT19] (ab233279)
    • Biotin Anti-CD19 antibody [LT19] (ab239237)
    • PerCP Anti-CD19 antibody [LT19] (ab239301)
    • PE/Cy7® Anti-CD19 antibody [LT19] (ab239315)
  • Isotype control

    • APC Mouse IgG1 [1F8] - Isotype Control (ab91358)

Applications

  • Application notes
    Flow Cyt: Use 20µl for 100µl human blood cells.
    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
    • Involvement in disease

      Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
    • Sequence similarities

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications

      Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P15391 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 930 Human
      • Omim: 107265 Human
      • SwissProt: P15391 Human
      • Unigene: 652262 Human
      • Alternative names

        • Antibody deficiency due to defect in CD19 antibody
        • Antibody deficiency due to defect in CD19, included antibody
        • AW495831 antibody
        • B lymphocyte antigen CD19 antibody
        • B lymphocyte surface antigen B4 antibody
        • B-lymphocyte antigen CD19 antibody
        • B-lymphocyte surface antigen B4 antibody
        • B4 antibody
        • CD19 antibody
        • CD19 antigen antibody
        • CD19 molecule antibody
        • Cd19 protein antibody
        • CD19_HUMAN antibody
        • CVID3 antibody
        • Differentiation antigen CD19 antibody
        • Leu 12 antibody
        • Leu-12 antibody
        • Leu12 antibody
        • MGC109570 antibody
        • MGC12802 antibody
        • T-cell surface antigen Leu-12 antibody
        see all

      Protocols

      • Flow cytometry protocols
      • Immunoprecipitation protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (2)

      Publishing research using ab18224? Please let us know so that we can cite the reference in this datasheet.

      ab18224 has been referenced in 2 publications.

      • Liu L  et al. Pfit is a structurally novel Crohn's disease-associated superantigen. PLoS Pathog 9:e1003837 (2013). PubMed: 24385909
      • Trepel M  et al. Phenotypic detection of clonotypic B cells in multiple myeloma by specific immunoglobulin ligands reveals their rarity in multiple myeloma. PLoS One 7:e31998 (2012). Flow Cyt ; Human . PubMed: 22384124

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Dear all,
      or a flow cytometry approach I need an anti human CD19 antibody.
      I have found the ab18224, but the problem is you do not have the corresponding isotype control for this? Is this correct?

      Read More

      Abcam community

      Verified customer

      Asked on Aug 28 2013

      Answer



      Ab18224 is a suitable alternative to target CD19 with Flow Cytometry. I’ve conducted a search for an isotype control and found the following product that matches the requirements needed: https://www.abcam.com/mouse-igg1-1f8-allophycocyanin-isotype-control-ab91358.html.

      Read More

      Ariana Veiga

      Abcam Scientific Support

      Answered on Aug 28 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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