APC Anti-CD3 antibody [SK7] (ab242259)
![Flow Cytometry - APC Anti-CD3 antibody [SK7] (ab242259)](https://www.abcam.com/ps/products/242/ab242259/Images/ab242259-331260-anti-cd3-antibody-sk7-allophycocyanin-flow-cytometry.jpg "Flow Cytometry - APC Anti-CD3 antibody [SK7] (ab242259)")
Key features and details
- APC Mouse monoclonal [SK7] to CD3
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Overview
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Product name
APC Anti-CD3 antibody [SK7]
See all CD3 primary antibodies -
Description
APC Mouse monoclonal [SK7] to CD3 -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD3. Human Thymocytes.
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Positive control
- Flow Cyt: Human peripheral blood lymphocytes.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from TCS via affinity chromatography. -
Clonality
Monoclonal -
Clone number
SK7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab242259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. The antibody has been diluted for use at 5 µl per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 µl. The number of cells within a sample should be determined empirically, but typically ranges between 1 x 10e5 to 1 x 10e8 cells. |
Target
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Function
The CD3 complex mediates signal transduction. -
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
Sequence similarities
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Omim: 186740 Human
- Omim: 186780 Human
- Omim: 186790 Human
- Omim: 186830 Human
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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Flow Cytometry - APC Anti-CD3 antibody [SK7] (ab242259)
Flow Cytometric analysis of human peripheral blood lymphocyte cells labeling CD3 with 5 µl (0.5 µg) of ab242259 (solid line) or 0.5 µg APC-Cyanine7 Mouse IgG1 isotype control (Dashed line).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab242259 has not yet been referenced specifically in any publications.
