For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    apc-cd3-epsilon-antibody-okt3-ab210315.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Adaptive Immunity T Cells CD
Share by email

APC Anti-CD3 epsilon antibody [OKT3] (ab210315)

  • Datasheet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Flow Cytometry - APC Anti-CD3 epsilon antibody [OKT3] (ab210315)

    Key features and details

    • APC Mouse monoclonal [OKT3] to CD3 epsilon
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: APC. Ex: 645nm, Em: 660nm
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 488 Alexa Fluor® 647 Alexa Fluor® 700 Biotin Carrier Free FITC redFluor™ 710 Unconjugated

    You may also be interested in

    Protein
    Product image
    Recombinant human CD3 epsilon protein (Active) (ab167755)
    Primary
    Product image
    Biotin Anti-CD3 antibody [UCHT1] (ab191112)
    Primary
    Anti-CD3 epsilon antibody [OKT3] - Low endotoxin, Azide free (ab210317)

    View more associated products

    Overview

    • Product name

      APC Anti-CD3 epsilon antibody [OKT3]
      See all CD3 epsilon primary antibodies
    • Description

      APC Mouse monoclonal [OKT3] to CD3 epsilon
    • Host species

      Mouse
    • Conjugation

      APC. Ex: 645nm, Em: 660nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Chimpanzee
    • Positive control

      • Human peripheral blood lymphocyte
    • General notes

      This antibody is a widely used phenotypic marker for human T cells. In addition, as the CD3 epsilon subunit of the TCR complex contains intracellular signaling domains, binding of ab210315 to CD3 epsilon can induce cell activation. 

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.1% Gelatin, 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      This antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
    • Clonality

      Monoclonal
    • Clone number

      OKT3
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Stem Cells
      • Hematopoietic Progenitors
      • Hematopoietic Stem Cells
      • Murine Lineage Negative
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage

    Associated products

    • Alternative Versions

      • FITC Anti-CD3 epsilon antibody [OKT3] (ab210316)
      • Anti-CD3 epsilon antibody [OKT3] - Low endotoxin, Azide free (ab210317)
      • redFluor™ 710 Anti-CD3 antibody [OKT3] (ab243296)
    • Isotype control

      • APC Mouse IgG2a [B12/8] - Isotype Control (ab91364)
    • Recombinant Protein

      • Recombinant human CD3 epsilon protein (Active) (ab167755)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab210315 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 5µl for 105-8 cells.

    in 100 μl volume.

    Notes
    Flow Cyt
    Use 5µl for 105-8 cells.

    in 100 μl volume.

    Target

    • Function

      The CD3 complex mediates signal transduction.
    • Sequence similarities

      Contains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P07766 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 742330 Chimpanzee
      • Entrez Gene: 916 Human
      • Omim: 186830 Human
      • SwissProt: P07766 Human
      • Unigene: 3003 Human
      • Alternative names

        • CD3 epsilon antibody
        • CD3e antibody
        • CD3e antigen antibody
        • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
        • CD3E antigen epsilon polypeptide antibody
        • CD3E antigen, epsilon subunit antibody
        • CD3e molecule epsilon antibody
        • CD3e molecule, epsilon (CD3 TCR complex) antibody
        • CD3e molecule, epsilon (CD3-TCR complex) antibody
        • CD3E_HUMAN antibody
        • IMD18 antibody
        • T cell antigen receptor complex epsilon subunit of T3 antibody
        • T cell surface antigen T3/Leu 4 epsilon chain antibody
        • T cell surface glycoprotein CD3 epsilon chain antibody
        • T-cell surface antigen T3/Leu-4 epsilon chain antibody
        • T-cell surface glycoprotein CD3 epsilon chain antibody
        • T3E antibody
        • TCRE antibody
        see all

      Images

      • Flow Cytometry - APC Anti-CD3 epsilon antibody [OKT3] (ab210315)
        Flow Cytometry - APC Anti-CD3 epsilon antibody [OKT3] (ab210315)

        Flow cytometry analysis of Human peripheral blood lymphocyte labeling CD3 epsilon using 5 μl (0.125 μg) of ab210315 (solid line) and 0.125 μg of Allophycocyanin Mouse IgG2a control (dashed line).

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab210315? Please let us know so that we can cite the reference in this datasheet.

      ab210315 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab210315.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2023 Abcam plc. All rights reserved.