APC Anti-CD3 epsilon antibody [OKT3] (ab210315)
![Flow Cytometry - APC Anti-CD3 epsilon antibody [OKT3] (ab210315)](https://www.abcam.com/ps/products/210/ab210315/Images/ab210315-256478-anti-cd3-epsilon-antibody-okt3-allophycocyanin-flow-cytometry.jpg "Flow Cytometry - APC Anti-CD3 epsilon antibody [OKT3] (ab210315)")
Key features and details
- APC Mouse monoclonal [OKT3] to CD3 epsilon
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
APC Anti-CD3 epsilon antibody [OKT3]
See all CD3 epsilon primary antibodies -
Description
APC Mouse monoclonal [OKT3] to CD3 epsilon -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee
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Positive control
- Human peripheral blood lymphocyte
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General notes
This antibody is a widely used phenotypic marker for human T cells. In addition, as the CD3 epsilon subunit of the TCR complex contains intracellular signaling domains, binding of ab210315 to CD3 epsilon can induce cell activation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.1% Gelatin, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
This antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
OKT3 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 5µl for 105-8 cells.
in 100 μl volume. |
| Notes |
|---|
|
Flow Cyt
Use 5µl for 105-8 cells. in 100 μl volume. |
Target
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Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 742330 Chimpanzee
- Entrez Gene: 916 Human
- Omim: 186830 Human
- SwissProt: P07766 Human
- Unigene: 3003 Human
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Alternative names
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
Images
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Flow Cytometry - APC Anti-CD3 epsilon antibody [OKT3] (ab210315)
Flow cytometry analysis of Human peripheral blood lymphocyte labeling CD3 epsilon using 5 μl (0.125 μg) of ab210315 (solid line) and 0.125 μg of Allophycocyanin Mouse IgG2a control (dashed line).
Datasheets and documents
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Datasheet download
References (0)
ab210315 has not yet been referenced specifically in any publications.
