APC Anti-CD35 antibody [E11] (ab243085)
![Flow Cytometry - APC Anti-CD35 antibody [E11] (ab243085)](https://www.abcam.com/ps/products/243/ab243085/Images/ab243085-332392-85j.jpg "Flow Cytometry - APC Anti-CD35 antibody [E11] (ab243085)")
Key features and details
- APC Mouse monoclonal [E11] to CD35
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Overview
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Product name
APC Anti-CD35 antibody [E11]
See all CD35 primary antibodies -
Description
APC Mouse monoclonal [E11] to CD35 -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates
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Immunogen
Tissue, cells or virus corresponding to Human CD35. Acute monocytic leukemia cells and normal blood monocytes.
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Positive control
- Flow Cyt: Human peripheral blood.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. -
Clonality
Monoclonal -
Clone number
E11 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab243085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. Or 100 µl of whole blood. |
Target
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Function
Mediates cellular binding of particles and immune complexes that have activated complement. -
Tissue specificity
Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. -
Sequence similarities
Belongs to the receptors of complement activation (RCA) family.
Contains 30 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1378 Human
- Omim: 120620 Human
- SwissProt: P17927 Human
- Unigene: 334019 Human
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Alternative names
- C3 binding protein antibody
- C3b/C4b receptor antibody
- C3BR antibody
see all
Images
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Flow Cytometry - APC Anti-CD35 antibody [E11] (ab243085)
Flow Cytometric analysis of human peripheral blood staining CD35 using ab243085. Surface staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab243085 has not yet been referenced specifically in any publications.
