Key features and details
- APC Mouse monoclonal [MEM-241] to CD4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Product nameAPC Anti-CD4 antibody [MEM-241]
See all CD4 primary antibodies
DescriptionAPC Mouse monoclonal [MEM-241] to CD4
ConjugationAPC. Ex: 645nm, Em: 660nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Fusion protein (Human).
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA
Concentration information loading...
PurityProtein A purified
- FITC Anti-CD4 antibody [MEM-241] (ab18281)
- PE Anti-CD4 antibody [MEM-241] (ab18282)
- APC/Cy7® Anti-CD4 antibody [MEM241] (ab233298)
- PE/Cy7® Anti-CD4 antibody [MEM241] (ab233660)
- Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)
- Biotin Anti-CD4 antibody [MEM-241] (ab28069)
- PerCP Anti-CD4 antibody [MEM-241] (ab65951)
Our Abpromise guarantee covers the use of ab18280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
- Information by UniProt
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
Surface staining of human peripheral blood cells with anti-human CD4 (MEM-241) APC.
Overlay histogram showing peripheral blood lymphocytes stained with ab18280 (red line). The cells were incubated with the antibody (ab18280, 1/20 dilution) for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) with secondary antibody DyLight® 649 goat anti-mouse IgG (H&L) (ab96882) at 1/200 dilution for 30 min at 4ºC. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.
ab18280 has not yet been referenced specifically in any publications.