APC Anti-CD41 antibody [MEM-06] (ab55866)
![Flow Cytometry - APC Anti-CD41 antibody [MEM-06] (ab55866)](https://www.abcam.com/ps/products/55/ab55866/Images/ab55866-287960-anti-cd41-antibody-mem-06-allophycocyanin-flow-cytometry.jpg "Flow Cytometry - APC Anti-CD41 antibody [MEM-06] (ab55866)")
Key features and details
- APC Mouse monoclonal [MEM-06] to CD41
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Overview
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Product name
APC Anti-CD41 antibody [MEM-06]
See all CD41 primary antibodies -
Description
APC Mouse monoclonal [MEM-06] to CD41 -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD41. Leukocytes of patient suffering from LGL-type leukaemia.
Database link: P08514 -
Positive control
- Flow Cyt: Human platelets.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
MEM-06 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PE Anti-CD41 antibody [MEM-06], prediluted (ab134372)
- PerCP Anti-CD41 antibody [MEM-06], prediluted (ab134373)
- FITC Anti-CD41 antibody [MEM-06] (ab21851)
- Anti-CD41 antibody [MEM-06] (ab2211)
- PE/Cy5® Anti-CD41 antibody [MEM-06] (ab233450)
- PE/Cy7® Anti-CD41 antibody [MEM-06] (ab239313)
- Alexa Fluor® 700 Anti-CD41 antibody [MEM-06] (ab270670)
- Biotin Anti-CD41 antibody [MEM-06] (ab28065)
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab55866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. Use 10µl for 106 cells in a suspension, or 100µl of whole blood. The content of a vial (1 ml) is sufficient for 100 tests. ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. -
Tissue specificity
Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells. -
Involvement in disease
Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3674 Human
- Omim: 607759 Human
- SwissProt: P08514 Human
- Unigene: 411312 Human
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Alternative names
- antigen CD41 antibody
- BDPLT16 antibody
- BDPLT2 antibody
see all
Images
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Flow Cytometry - APC Anti-CD41 antibody [MEM-06] (ab55866)
Surface staining of human platelets with ab55866.
References (0)
ab55866 has not yet been referenced specifically in any publications.
