APC Anti-CD41 antibody [MWReg30] (ab95725)
![Flow Cytometry - APC Anti-CD41 antibody [MWReg30] (ab95725)](https://www.abcam.com/ps/products/95/ab95725/Images/ab95725-109976-anti-cd41-antibody-mwreg30-allophycocyanin-flow-cytometry.jpg "Flow Cytometry - APC Anti-CD41 antibody [MWReg30] (ab95725)")
Key features and details
- APC Rat monoclonal [MWReg30] to CD41
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Overview
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Product name
APC Anti-CD41 antibody [MWReg30]
See all CD41 primary antibodies -
Description
APC Rat monoclonal [MWReg30] to CD41 -
Host species
Rat -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Mouse platelets
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Positive control
- Mouse platelets
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.1% Gelatin, PBS
Containing horse serum -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MWReg30 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab95725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.125-0.25µg for 105-8 cells. A final volume of 100µl is recommended.
ab154433 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. -
Tissue specificity
Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells. -
Involvement in disease
Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16399 Mouse
- SwissProt: Q9QUM0 Mouse
- Unigene: 26646 Mouse
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Alternative names
- antigen CD41 antibody
- BDPLT16 antibody
- BDPLT2 antibody
see all
Images
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Flow Cytometry - APC Anti-CD41 antibody [MWReg30] (ab95725)Staining of mouse platelets with anti-Mouse CD61 PE and 0.125µg of Rat IgG1 ? Isotype Control APC (left) or 0.125 µg of ab95725 (right).
Datasheets and documents
References (4)
ab95725 has been referenced in 4 publications.
- Bertrand JY et al. Characterization of purified intraembryonic hematopoietic stem cells as a tool to define their site of origin. Proc Natl Acad Sci U S A 102:134-9 (2005). Flow Cyt . PubMed: 15623562
- Mitjavila-Garcia MT et al. Expression of CD41 on hematopoietic progenitors derived from embryonic hematopoietic cells. Development 129:2003-13 (2002). Flow Cyt . PubMed: 11934866
- Teeling JL et al. Therapeutic efficacy of intravenous immunoglobulin preparations depends on the immunoglobulin G dimers: studies in experimental immune thrombocytopenia. Blood 98:1095-9 (2001). PubMed: 11493456
- Nieswandt B et al. Acute systemic reaction and lung alterations induced by an antiplatelet integrin gpIIb/IIIa antibody in mice. Blood 94:684-93 (1999). Flow Cyt . PubMed: 10397735
