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  1. Link

    apc-cd5-antibody-53-73-ab218754.pdf

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Immunology Adaptive Immunity B Cells CD
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APC Anti-CD5 antibody [53-7.3] (ab218754)

  • Datasheet
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Flow Cytometry - APC Anti-CD5 antibody [53-7.3] (ab218754)

    Key features and details

    • APC Rat monoclonal [53-7.3] to CD5
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: APC. Ex: 645nm, Em: 660nm
    • Isotype: IgG2a

    Overview

    • Product name

      APC Anti-CD5 antibody [53-7.3]
      See all CD5 primary antibodies
    • Description

      APC Rat monoclonal [53-7.3] to CD5
    • Host species

      Rat
    • Conjugation

      APC. Ex: 645nm, Em: 660nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • C57Bl/6 splenocytes.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      ab218754 was purified from tissue culture supernatant and conjugated under optimal conditions, with unreacted dye removed from the preparation.
    • Clonality

      Monoclonal
    • Clone number

      53-7.3
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Adaptive Immunity
      • B Cells
      • CD
      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage

    Associated products

    • Alternative Versions

      • FITC Anti-CD5 antibody [53-7.3] (ab114048)
      • PE Anti-CD5 antibody [53-7.3] (ab114078)
      • PE Anti-CD5 antibody [53-7.3] (ab233464)
      • Anti-CD5 antibody [53-7.3] (ab234191)
    • Recombinant Protein

      • Recombinant Human CD5 protein (denatured) (ab180322)

    Applications

    Our Abpromise guarantee covers the use of ab218754 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.

    Use 0.25 μg per test

    Target

    • Function

      May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.
    • Sequence similarities

      Contains 3 SRCR domains.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P06127 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 12507 Mouse
      • SwissProt: P13379 Mouse
      • Unigene: 779 Mouse
      • Alternative names

        • CD 5 antibody
        • CD5 antibody
        • CD5 antigen (p56 62) antibody
        • CD5 antigen antibody
        • CD5 molecule antibody
        • CD5_HUMAN antibody
        • LEU1 antibody
        • LyA antibody
        • Lymphocyte antigen T1/Leu-1 antibody
        • OTTHUMP00000236973 antibody
        • T-cell surface glycoprotein CD5 antibody
        • T1 antibody
        see all

      Images

      • Flow Cytometry - APC Anti-CD5 antibody [53-7.3] (ab218754)
        Flow Cytometry - APC Anti-CD5 antibody [53-7.3] (ab218754)

        Flow cytometric analysis of C57Bl/6 splenocytes labeling CD5 with 0.25 μg of ab218754 (solid line) or 0.25 μg of Allophycocyanin Rat IgG2a isotype control (dashed line).

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab218754? Please let us know so that we can cite the reference in this datasheet.

      ab218754 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab218754.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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