APC Anti-CD69 antibody [FN50] (ab95609)
Key features and details
- APC Mouse monoclonal [FN50] to CD69
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Overview
-
Product name
APC Anti-CD69 antibody [FN50]
See all CD69 primary antibodies -
Description
APC Mouse monoclonal [FN50] to CD69 -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Stimulated Human B lymphocytes
-
Positive control
- 3 day PHA activated Human peripheral blood leukocytes; Resting and 6 hour TPA activated Human PBMC
-
General notes
Excitation: 633 nm Emission: 660 nm.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 0.87% Sodium chloride, PBS -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
FN50 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
- PE/Cy7® Anti-CD69 antibody [FN50] (ab135930)
- FITC Anti-CD69 antibody [FN50], prediluted (ab135931)
- PE Anti-CD69 antibody [FN50], prediluted (ab135932)
- PerCP Anti-CD69 antibody [FN50], prediluted (ab135933)
- Alexa Fluor® 647 Anti-CD69 antibody [FN50] (ab187772)
- PE/Cy7® Anti-CD69 antibody [FN50] (ab233573)
- APC Anti-CD69 antibody [FN50] (ab239281)
- Anti-CD69 antibody [FN50] (ab51862)
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt | Use 5µl for 106 cells. ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
-
Function
Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. -
Tissue specificity
Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets. -
Sequence similarities
Contains 1 C-type lectin domain. -
Developmental stage
Earliest inducible cell surface glycoprotein acquired during lymphoid activation. -
Post-translational
modificationsConstitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 969 Human
- Omim: 107273 Human
- SwissProt: Q07108 Human
- Unigene: 208854 Human
-
Alternative names
- Activation inducer molecule (AIM/CD69) antibody
- Activation inducer molecule antibody
- AIM antibody
see all
Images
-
Flow cytometry staining of 3-day PHA activated Human peripheral blood leukocytes with APC Mouse IgG1 kappa Isotype Control (open histogram) or ab95609 (colored histogram). Total viable cells were used for analysis.
-
Flow Cytometry - APC Anti-CD69 antibody [FN50] (ab95609)This image is courtesy of an anonymous AbreviewFlow cytometry analysis of Cynomolgus monkey whole blood after red blood cell lysis, staining CD69 with ab95609.
Cells were stimulated with PMA and red blood cells were lysed. Samples were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C.
Gating Strategy: Live lymphocytes
Datasheets and documents
References (0)
ab95609 has not yet been referenced specifically in any publications.