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  1. Link

    apc-cd8-alpha-antibody-53-67-ab25499.pdf

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Immunology Adaptive Immunity T Cells Cytotoxic Cells
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APC Anti-CD8 alpha antibody [53-6.7] (ab25499)

  • Datasheet
Submit a review Q&A (1)References (2)

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Abpromise

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Flow Cytometry - APC Anti-CD8 alpha antibody [53-6.7] (ab25499)

    Key features and details

    • APC Rat monoclonal [53-6.7] to CD8 alpha
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: APC. Ex: 645nm, Em: 660nm
    • Isotype: IgG2a

    You may also be interested in

    Protein
    Product image
    Recombinant Human CD8 alpha protein (ab114161)

    View more associated products

    Overview

    • Product name

      APC Anti-CD8 alpha antibody [53-6.7]
      See all CD8 alpha primary antibodies
    • Description

      APC Rat monoclonal [53-6.7] to CD8 alpha
    • Host species

      Rat
    • Conjugation

      APC. Ex: 645nm, Em: 660nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      The details of the immunogen for this antibody are not available.

    • General notes

      This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
    • Concentration information loading...
    • Purity

      Affinity purified
    • Primary antibody notes

      This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
    • Clonality

      Monoclonal
    • Clone number

      53-6.7
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • Cytotoxic Cells
      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Immunology
      • Adaptive Immunity
      • MHC
      • Class I
      • Cancer
      • Tumor immunology
      • CD markers

    Associated products

    • Alternative Versions

      • Biotin Anti-CD8 alpha antibody [53-6.7] (ab25176)
      • PE/Texas Red® Anti-CD8 alpha antibody [53-6.7] (ab25294)
      • Anti-CD8 alpha antibody [53-6.7] (ab25478)
      • PE Anti-CD8 alpha antibody [53-6.7] (ab25498)
      • FITC Anti-CD8 alpha antibody [53-6.7] (ab25676)
      • PE Anti-CD8 alpha antibody [53-6.7] (ab272343)
      • Cy5 ® Anti-CD8 alpha antibody [53-6.7] (ab39850)
      • PE/Cy7® Anti-CD8 alpha antibody [53-6.7] (ab39853)
      • APC/Cy5.5® Anti-CD8 alpha antibody [53-6.7] (ab82005)
      • PE/Cy5® Anti-CD8 alpha antibody [53-6.7] (ab95588)
    • Recombinant Protein

      • Recombinant Human CD8 alpha protein (ab114161)

    Applications

    Our Abpromise guarantee covers the use of ab25499 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.

    ab129719 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
    • Involvement in disease

      Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications

      All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P01732 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 12525 Mouse
      • SwissProt: P01731 Mouse
      • Unigene: 1858 Mouse
      • Alternative names

        • alpha polypeptide (p32) antibody
        • CD_antigen=CD8a antibody
        • CD8 antibody
        • CD8 antigen alpha polypeptide antibody
        • CD8 antigen alpha polypeptide (p32) antibody
        • CD8 antigen, alpha polypeptide (p32) antibody
        • CD8a antibody
        • CD8A antigen antibody
        • CD8A molecule antibody
        • CD8A_HUMAN antibody
        • Leu2 antibody
        • Leu2 T lymphocyte antigen antibody
        • Ly 2 antibody
        • Ly 35 antibody
        • Ly B antibody
        • Ly2 antibody
        • Ly3 antibody
        • Ly35 antibody
        • LyB antibody
        • Lyt 2.1 lymphocyte differentiation antigen (AA at 100) antibody
        • LYT3 antibody
        • MAL antibody
        • OKT8 T cell antigen antibody
        • OTTHUMP00000160760 antibody
        • OTTHUMP00000160764 antibody
        • OTTHUMP00000203528 antibody
        • OTTHUMP00000203721 antibody
        • p32 antibody
        • T cell antigen Leu2 antibody
        • T cell co receptor antibody
        • T lymphocyte differentiation antigen T8/Leu 2 antibody
        • T-cell surface glycoprotein CD8 alpha chain antibody
        • T-cell surface glycoprotein Lyt 2 antibody
        • T-lymphocyte differentiation antigen T8/Leu-2 antibody
        • T8 T cell antigen antibody
        • T8/Leu-2 T-lymphocyte differentiation antigen antibody
        see all

      Images

      • Flow Cytometry - APC Anti-CD8 alpha antibody [53-6.7] (ab25499)
        Flow Cytometry - APC Anti-CD8 alpha antibody [53-6.7] (ab25499)

        Flow Cytometry analysis of C57Bl/6 splenocytes labeling CD8 alpha with ab25499 at 0.25 µg (solid line), and Rat IgG2a (Allophycocyanin) isotype control at 0.25 µg (dashed line).

      Protocols

      • Flow cytometry protocols
      • Immunoprecipitation protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (2)

      Publishing research using ab25499? Please let us know so that we can cite the reference in this datasheet.

      ab25499 has been referenced in 2 publications.

      • Zhu G  et al. ShenQi FuZheng Injection ameliorates fatigue-like behavior in mouse models of cancer-related fatigue. Biomed Pharmacother 111:1376-1382 (2019). PubMed: 30841452
      • Anraku M  et al. Synergistic antitumor effects of regulatory T cell blockade combined with pemetrexed in murine malignant mesothelioma. J Immunol 185:956-66 (2010). Flow Cyt ; Mouse . PubMed: 20548032

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Dear abcam support desk, Thankyou so much for your fast response. I’mwondering if you still sell this item ab52854 because this antibody works great onour mouse section. Ifnot, do you have any recommendation? Let me know. Thank You for your help.  

      Read More

      Abcam community

      Verified customer

      Asked on Aug 22 2011

      Answer

      Thank you for contacting us. We have many other products that works well on mouse tissues and are fully tested. The catalogue numbers are Un conjugated anti CD8 antibodies ab22378 ab25478 ab3081 ab108292 Conjugated anti CD8 antibodies ab25294 ab25676 ab60077 ab25499 ab25498 ab39853 These antibodies will work on mouse section in the applications as recommended on datasheet. Please check the datasheets and buy the antibody as per your requirements. All antibodies are equally good and are fully guaranteed under Abpromise to work with mouse samples. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Aug 22 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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