APC Anti-CD8 alpha antibody [53-6.7] (ab25499)
![Flow Cytometry - APC Anti-CD8 alpha antibody [53-6.7] (ab25499)](https://www.abcam.com/ps/products/25/ab25499/Images/ab25499-277205-anti-cd8-alpha-antibody-53-67-allophycocyanin-flow-cytometry.png "Flow Cytometry - APC Anti-CD8 alpha antibody [53-6.7] (ab25499)")
Key features and details
- APC Rat monoclonal [53-6.7] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG2a
Overview
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Product name
APC Anti-CD8 alpha antibody [53-6.7]
See all CD8 alpha primary antibodies -
Description
APC Rat monoclonal [53-6.7] to CD8 alpha -
Host species
Rat -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Primary antibody notes
This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. -
Clonality
Monoclonal -
Clone number
53-6.7 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Biotin Anti-CD8 alpha antibody [53-6.7] (ab25176)
- PE/Texas Red® Anti-CD8 alpha antibody [53-6.7] (ab25294)
- Anti-CD8 alpha antibody [53-6.7] (ab25478)
- PE Anti-CD8 alpha antibody [53-6.7] (ab25498)
- FITC Anti-CD8 alpha antibody [53-6.7] (ab25676)
- PE Anti-CD8 alpha antibody [53-6.7] (ab272343)
- Cy5 ® Anti-CD8 alpha antibody [53-6.7] (ab39850)
- PE/Cy7® Anti-CD8 alpha antibody [53-6.7] (ab39853)
- APC/Cy5.5® Anti-CD8 alpha antibody [53-6.7] (ab82005)
- PE/Cy5® Anti-CD8 alpha antibody [53-6.7] (ab95588)
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab25499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. ab129719 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
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Alternative names
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
Images
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Flow Cytometry - APC Anti-CD8 alpha antibody [53-6.7] (ab25499)
Flow Cytometry analysis of C57Bl/6 splenocytes labeling CD8 alpha with ab25499 at 0.25 µg (solid line), and Rat IgG2a (Allophycocyanin) isotype control at 0.25 µg (dashed line).
Protocols
Datasheets and documents
References (2)
ab25499 has been referenced in 2 publications.
- Zhu G et al. ShenQi FuZheng Injection ameliorates fatigue-like behavior in mouse models of cancer-related fatigue. Biomed Pharmacother 111:1376-1382 (2019). PubMed: 30841452
- Anraku M et al. Synergistic antitumor effects of regulatory T cell blockade combined with pemetrexed in murine malignant mesothelioma. J Immunol 185:956-66 (2010). Flow Cyt ; Mouse . PubMed: 20548032
