APC Anti-CD8 antibody [MEM-31] (ab26004)
Key features and details
- APC Mouse monoclonal [MEM-31] to CD8
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG2a
Overview
-
Product name
APC Anti-CD8 antibody [MEM-31]
See all CD8 primary antibodies -
Description
APC Mouse monoclonal [MEM-31] to CD8 -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Species reactivity
Reacts with: Human -
Immunogen
Tissue/ cell preparation: Crude thymus membrane fraction. (Human).
-
Epitope
This antibody recognizes a conformationally dependent epitope of CD8 antigen. -
General notes
The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: 0.2% BSA -
Concentration information loading... -
Purification notes
The conjugate is purified by size exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
MEM-31 -
Isotype
IgG2a -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
Target
-
Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 925 Human
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human
-
Form
CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation. -
Alternative names
- CD8 antibody
- CD8 antigen, alpha polypeptide (p32) antibody
- CD8a antibody
see all
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab26004 has been referenced in 2 publications.
- Dieckmann NM et al. Munc18-2 is required for Syntaxin 11 Localization on the Plasma Membrane in Cytotoxic T-Lymphocytes. Traffic 16:1330-41 (2015). PubMed: 26771955
- Horejsí V et al. Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha) 32:12-25 (1986). PubMed: 2422063