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  1. Link

    apc-cd80-antibody-mem-233-ab27554.pdf

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Immunology Adaptive Immunity B Cells CD
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APC Anti-CD80 antibody [MEM-233] (ab27554)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (2)

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Key features and details

  • APC Mouse monoclonal [MEM-233] to CD80
  • Reacts with: Human
  • Conjugation: APC. Ex: 645nm, Em: 660nm
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human CD80 protein (ab131745)

View more associated products

Overview

  • Product name

    APC Anti-CD80 antibody [MEM-233]
    See all CD80 primary antibodies
  • Description

    APC Mouse monoclonal [MEM-233] to CD80
  • Host species

    Mouse
  • Conjugation

    APC. Ex: 645nm, Em: 660nm
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Human CD80 fusion protein.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituent: 0.2% BSA

    High grade protease free BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    ab27554 was purified by size exclusion chromatography.
  • Clonality

    Monoclonal
  • Clone number

    MEM-233
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage

Associated products

  • Alternative Versions

    • FITC Anti-CD80 antibody [MEM-233] (ab18279)
    • PE/Cy5® Anti-CD80 antibody [MEM-233] (ab233572)
    • PE/Cy7® Anti-CD80 antibody [MEM-233] (ab234229)
    • Alexa Fluor® 700 Anti-CD80 antibody [MEM-233] (ab270668)
    • PE Anti-CD80 antibody [MEM-233], prediluted (ab69778)
    • Anti-CD80 antibody [MEM-233] (ab8239)
    • PerCP Anti-CD80 antibody [MEM-233] (ab82410)
  • Isotype control

    • APC Mouse IgG1 [1F8] - Isotype Control (ab91358)
  • Recombinant Protein

    • Recombinant Human CD80 protein (ab131745)

Target

  • Function

    Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
  • Tissue specificity

    Expressed on activated B-cells, macrophages and dendritic cells.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P33681 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 941 Human
    • Omim: 112203 Human
    • SwissProt: P33681 Human
    • Unigene: 838 Human
    • Alternative names

      • Activation B7-1 antigen antibody
      • B lymphocyte activation antigen B7 antibody
      • B7 antibody
      • B7-1 antibody
      • B7-1 antigen antibody
      • B7.1 antibody
      • BB1 antibody
      • CD28 antigen ligand 1 antibody
      • CD28LG antibody
      • CD28LG1 antibody
      • CD80 antibody
      • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody
      • CD80 antigen antibody
      • CD80 molecule antibody
      • CD80_HUMAN antibody
      • Costimulatory factor CD80 antibody
      • costimulatory molecule variant IgV-CD80 antibody
      • CTLA-4 counter-receptor B7.1 antibody
      • LAB7 antibody
      • T-lymphocyte activation antigen CD80 antibody
      see all

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab27554? Please let us know so that we can cite the reference in this datasheet.

    ab27554 has been referenced in 2 publications.

    • Kew VG  et al. LPS promotes a monocyte phenotype permissive for human cytomegalovirus immediate-early gene expression upon infection but not reactivation from latency. Sci Rep 7:810 (2017). Flow Cyt ; Human . PubMed: 28400599
    • Zhang J  et al. Recombinant Murine Gamma Herpesvirus 68 Carrying KSHV G Protein-Coupled Receptor Induces Angiogenic Lesions in Mice. PLoS Pathog 11:e1005001 (2015). IHC ; Human . PubMed: 26107716

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Flow Cytometry abreview for Anti-CD80 antibody [MEM-233] (Allophycocyanin)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    Formaldehyde
    Permeabilization
    No
    Sample
    Human Cell (PBMC (monocyte depleted))
    Specification
    PBMC (monocyte depleted)
    Gating Strategy
    lymphocytes
    Preparation
    Cell harvesting/tissue preparation method: Ficoll
    Sample buffer: PBX
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 10 2014

    Flow Cytometry abreview for Anti-CD80 antibody [MEM-233] (Allophycocyanin)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Activated CD19+ B-cells)
    Specification
    Activated CD19+ B-cells
    Preparation
    Cell harvesting/tissue preparation method: EBV transformed / activated B-cells cultured for 3 days in RPMI 1640, 10 % FCS
    Sample buffer: FACS buffer (PBS, 0.5% FCS, 0.5M EDTA)
    Fixation
    None
    Permeabilization
    No
    Gating Strategy
    CD19+ B-cells
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 27 2009

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