APC Anti-CD86 antibody [BU63], prediluted (ab134385)
![Flow Cytometry - APC Anti-CD86 antibody [BU63], prediluted (ab134385)](https://www.abcam.com/ps/products/134/ab134385/Images/ab134385-278861-anti-cd86-antibody-bu63-prediluted-allophycocyanin-flow-cytometry.jpg "Flow Cytometry - APC Anti-CD86 antibody [BU63], prediluted (ab134385)")
Key features and details
- APC Mouse monoclonal [BU63] to CD86, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Overview
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Product name
APC Anti-CD86 antibody [BU63], prediluted
See all CD86 primary antibodies -
Description
APC Mouse monoclonal [BU63] to CD86, prediluted -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
B-lymphoblastoid cell line ARH 77.
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Positive control
- Human whole blood.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The antibody is purified by size-exclusion chromatography and adjusted for direct use. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
BU63 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)
- PE/Cy7® Anti-CD86 antibody [BU63] (ab233571)
- Anti-CD86 antibody [BU63] (ab234000)
- PE/Cy5® Anti-CD86 antibody [BU63] (ab234226)
- PerCP Anti-CD86 antibody [BU63] (ab77131)
- PE Anti-CD86 antibody [BU63] (ab77226)
- FITC Anti-CD86 antibody [BU63] (ab77276)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. or 100µl whole blood |
Target
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Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
Tissue specificity
Expressed by activated B-lymphocytes and monocytes. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPolyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 942 Human
- Omim: 601020 Human
- SwissProt: P42081 Human
- Unigene: 171182 Human
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Alternative names
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
see all
Images
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Flow Cytometry - APC Anti-CD86 antibody [BU63], prediluted (ab134385)Flow Cytometry analysis of human peripheral blood cells labeling CD86 with Anti-CD86 antibody [BU63], prediluted (Allophycocyanin) (ab134385).
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![Flow Cytometry - APC Anti-CD86 antibody [BU63], prediluted (ab134385)](https://www.abcam.com/ps/products/134/ab134385/Images/ab134385-211725-anti-cd86-antibody-bu63-prediluted-allophycocyanin-flow-cytometry.jpg)
Flow Cytometry - APC Anti-CD86 antibody [BU63], prediluted (ab134385)This image is courtesy of an anonymous Abreviewab134385 staining CD86 in Human PBMCs by Flow Cytometry. The sample was incubated with the primary antibody (1/50 in 2% Human serum + 0.5mM EDTA in PBS) for 25 minutes at 4°C.
Gating Strategy: Lymphocytes.
Protocols
References (0)
ab134385 has not yet been referenced specifically in any publications.
