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  1. Link

    apc-cd90--thy1-antibody-5e10-ab139364.pdf

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Immunology Adaptive Immunity T Cells CD
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APC Anti-CD90 / Thy1 antibody [5E10] (ab139364)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (8)

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Flow Cytometry - APC Anti-CD90 / Thy1 antibody [5E10] (ab139364)

    Key features and details

    • APC Mouse monoclonal [5E10] to CD90 / Thy1
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: APC. Ex: 645nm, Em: 660nm
    • Isotype: IgG1

    You may also be interested in

    Protein
    Recombinant Human CD90 / Thy1 protein (Fc Chimera) (ab157072)

    View more associated products

    Overview

    • Product name

      APC Anti-CD90 / Thy1 antibody [5E10]
      See all CD90 / Thy1 primary antibodies
    • Description

      APC Mouse monoclonal [5E10] to CD90 / Thy1
    • Host species

      Mouse
    • Conjugation

      APC. Ex: 645nm, Em: 660nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Human erythroleukemia cell line cell preparation.

    • Positive control

      • Flow Cyt: Jurkat cells.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.4
      Preservative: 0.1% Sodium azide
      Constituents: 99% PBS, 0.2% BSA

      BSA is high-grade protease free
    • Concentration information loading...
    • Purity

      Size exclusion
    • Clonality

      Monoclonal
    • Clone number

      5E10
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage

    Associated products

    • Alternative Versions

      • Anti-CD90 / Thy1 antibody [5E10] (ab123511)
      • FITC Anti-CD90 / Thy1 antibody [5E10] (ab124527)
      • PerCP/Cy5.5® Anti-CD90 / Thy1 antibody [5E10] (ab134360)
      • Biotin Anti-CD90 / Thy1 antibody [5E10] (ab134361)
      • PE/Cy5® Anti-CD90 / Thy1 antibody [5E10] (ab269286)
      • PE/Cy5® Anti-CD90 / Thy1 antibody [5E10] (ab95698)
      • PE Anti-CD90 / Thy1 antibody [5E10] (ab95700)
    • Isotype control

      • APC Mouse IgG1 [1F8] - Isotype Control (ab91358)
    • Recombinant Protein

      • Recombinant Human CD90 / Thy1 protein (Fc Chimera) (ab157072)

    Applications

    Our Abpromise guarantee covers the use of ab139364 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.

    10 μl reagent / 100 μl of whole blood or 106 cells in a suspension.

    Target

    • Function

      May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P04216 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7070 Human
      • Omim: 188230 Human
      • SwissProt: P04216 Human
      • Unigene: 644697 Human
      • Alternative names

        • CD7 antibody
        • CD90 antibody
        • CD90 antigen antibody
        • CDw90 antibody
        • FLJ33325 antibody
        • MGC128895 antibody
        • T25 antibody
        • Theta antigen antibody
        • Thy 1 antibody
        • Thy 1 cell surface antigen antibody
        • Thy 1 membrane glycoprotein antibody
        • Thy 1 T cell antigen antibody
        • Thy 1.2 antibody
        • Thy-1 antigen antibody
        • Thy-1 membrane glycoprotein antibody
        • Thy1 antibody
        • Thy1 antigen antibody
        • Thy1 T cell antigen antibody
        • Thy1.1 antibody
        • Thy1.2 antibody
        • THY1_HUMAN antibody
        • Thymus cell antigen 1, theta antibody
        see all

      Images

      • Flow Cytometry - APC Anti-CD90 / Thy1 antibody [5E10] (ab139364)
        Flow Cytometry - APC Anti-CD90 / Thy1 antibody [5E10] (ab139364)
        Flow cytometry analysis (surface staining) of Jurkat cells (red) and SP2 cells (blue) labeling CD90 with ab139364.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (8)

      Publishing research using ab139364? Please let us know so that we can cite the reference in this datasheet.

      ab139364 has been referenced in 8 publications.

      • Zhu S  et al. Bidirectional ephrinB2-EphB4 signaling regulates the osteogenic differentiation of canine periodontal ligament stem cells. Int J Mol Med 45:897-909 (2020). PubMed: 31985015
      • Tugnoli B  et al. Butyric acid induces spontaneous adipocytic differentiation of porcine bone marrow-derived mesenchymal stem cells. In Vitro Cell Dev Biol Anim 55:17-24 (2019). PubMed: 30456456
      • Dothel G  et al. Ex vivo effect of vascular wall stromal cells secretome on enteric ganglia. World J Gastroenterol 25:4892-4903 (2019). PubMed: 31543681
      • Burmeister DM  et al. Delivery of Allogeneic Adipose Stem Cells in Polyethylene Glycol-Fibrin Hydrogels as an Adjunct to Meshed Autografts After Sharp Debridement of Deep Partial Thickness Burns. Stem Cells Transl Med 7:360-372 (2018). ICC/IF ; Pig . PubMed: 29457376
      • McDaniel JS  et al. Quantitative Assessment of Optimal Bone Marrow Site for the Isolation of Porcine Mesenchymal Stem Cells. Stem Cells Int 2017:1836960 (2017). Flow Cyt ; Pig . PubMed: 28539939
      • Zaniboni A  et al. In vitro differentiation of porcine aortic vascular precursor cells to endothelial and vascular smooth muscle cells. Am J Physiol Cell Physiol 309:C320-31 (2015). Flow Cyt ; Human . PubMed: 26135800
      • Baulier E  et al. Amniotic fluid-derived mesenchymal stem cells prevent fibrosis and preserve renal function in a preclinical porcine model of kidney transplantation. Stem Cells Transl Med 3:809-20 (2014). PubMed: 24797827
      • Zaniboni A  et al. Cells derived from porcine aorta tunica media show mesenchymal stromal-like cell properties in in vitro culture. Am J Physiol Cell Physiol 306:C322-33 (2014). Flow Cyt ; Pig . PubMed: 24304832

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Flow Cytometry abreview for Anti-CD90 / Thy1 antibody [5E10] (APC)

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Bos taurus Cell (Umbilical Cord Mesenchymal Stem Cells)
      Permeabilization
      No
      Gating Strategy
      Sample had one population of cells, gating includes 96.5% cells.
      Specification
      Umbilical Cord Mesenchymal Stem Cells
      Preparation
      Cell harvesting/tissue preparation method: Harvesting of cells by trypsination for 3 minutes
      Sample buffer: 10% chicken serum in PBS
      Fixation
      No fixation
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 01 2020

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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