APC Anti-DC-SIGN antibody [UW60.1] (ab180548)
![Flow Cytometry - APC Anti-DC-SIGN antibody [UW60.1] (ab180548)](https://www.abcam.com/ps/products/180/ab180548/Images/ab180548-279894-anti-dc-sign-antibody-uw601-allophycocyanin-flow-cytometry.jpg "Flow Cytometry - APC Anti-DC-SIGN antibody [UW60.1] (ab180548)")
Key features and details
- APC Mouse monoclonal [UW60.1] to DC-SIGN
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG2a
Overview
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Product name
APC Anti-DC-SIGN antibody [UW60.1]
See all DC-SIGN primary antibodies -
Description
APC Mouse monoclonal [UW60.1] to DC-SIGN -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein (His-tag) corresponding to Human DC-SIGN.
Database link: Q9NNX6 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
UW60.1 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab180548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. |
Target
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Function
Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells. -
Tissue specificity
Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes. -
Sequence similarities
Contains 1 C-type lectin domain. -
Domain
The tandem repeat domain, also called neck domain, mediates oligomerization. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 30835 Human
- Omim: 604672 Human
- SwissProt: Q9NNX6 Human
- Unigene: 278694 Human
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Alternative names
- C type lectin domain family 4 member L antibody
- C-type lectin domain family 4 member L antibody
- CD 209 antibody
see all
Images
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Flow Cytometry - APC Anti-DC-SIGN antibody [UW60.1] (ab180548)Surface staining of human monocytes-derived dendritic cells with anti-human CD209 (UW60.1) APC.
Datasheets and documents
References (0)
ab180548 has not yet been referenced specifically in any publications.
