RecombinantRabMAb

Recombinant APC Anti-DGCR8 antibody [EPR18757] (ab221302)

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Flow Cytometry - APC Anti-DGCR8 antibody [EPR18757] (ab221302)
  • APC Anti-DGCR8 antibody [EPR18757] (ab221302)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • APC Rabbit monoclonal [EPR18757] to DGCR8
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: APC. Ex: 645nm, Em: 660nm

Overview

  • Product name

    APC Anti-DGCR8 antibody [EPR18757]
    See all DGCR8 primary antibodies

  • Description

    APC Rabbit monoclonal [EPR18757] to DGCR8

  • Host species

    Rabbit

  • Conjugation

    APC. Ex: 645nm, Em: 660nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Human DGCR8 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8WYQ5

  • Positive control

    • Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab221302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

Target

  • Function

    Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal.

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 WW domain.

  • Domain

    Both DRBM domains are required for efficient binding to pri-miRNA. The region between residues 276 and 498 has an autoinhibitory function on pri-miRNA processing activity.

  • Cellular localization

    Nucleus. Nucleus > nucleolus. Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DGCRK6 antibody
    • C22orf12 antibody
    • D16H22S788E antibody
    • D16Wis2 antibody
    • DGCR 8 antibody
    • Dgcr8 antibody
    • DGCR8 microprocessor complex subunit antibody
    • DGCR8_HUMAN antibody
    • DGCRK 6 antibody
    • DiGeorge syndrome critical region 8 antibody
    • DiGeorge syndrome critical region gene 8 antibody
    • Gy1 antibody
    • Microprocessor complex subunit DGCR8 antibody
    • pasha antibody
    see all

Images

  • Flow Cytometry - APC Anti-DGCR8 antibody [EPR18757] (ab221302)
    Flow Cytometry - APC Anti-DGCR8 antibody [EPR18757] (ab221302)

    Overlay histogram showing HeLa cells stained with ab221302 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab221302, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • APC Anti-DGCR8 antibody [EPR18757] (ab221302)
    APC Anti-DGCR8 antibody [EPR18757] (ab221302)

References (0)

Publishing research using ab221302? Please let us know so that we can cite the reference in this datasheet.

ab221302 has not yet been referenced specifically in any publications.

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