APC Anti-F4/80 antibody [BM8.1] (ab210250)
![Flow Cytometry - APC Anti-F4/80 antibody [BM8.1] (ab210250)](https://www.abcam.com/ps/products/210/ab210250/Images/ab210250-256787-anti-f4-80-antibody-bm81-allophycocyanin-flow-cytometry.jpg "Flow Cytometry - APC Anti-F4/80 antibody [BM8.1] (ab210250)")
Key features and details
- APC Rat monoclonal [BM8.1] to F4/80
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG2a
Overview
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Product name
APC Anti-F4/80 antibody [BM8.1]
See all F4/80 primary antibodies -
Description
APC Rat monoclonal [BM8.1] to F4/80 -
Host species
Rat -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Mouse C57Bl/6 bone marrow cells
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General notes
ab210250 is widely used together with antibodies to CD115, CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.
The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
ab210250 was purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
BM8.1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab210250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. |
Target
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Function
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
Tissue specificity
Expression is restricted to eosinophils. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 13733 Mouse
- SwissProt: Q61549 Mouse
- Unigene: 2254 Mouse
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Alternative names
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
Images
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Flow Cytometry - APC Anti-F4/80 antibody [BM8.1] (ab210250)
Flow cytometric analysis of F4/80 in mouse C57Bl/6 bone marrow cells using FITC Anti-Mouse CD11b and 0.5 μg ab210250 (right panel), or 0.5 μg Allophycocyanin Rat IgG2a isotype control (left panel).
Datasheets and documents
References (1)
ab210250 has been referenced in 1 publication.
- Hanot Mambres D et al. In Situ Characterization of Splenic Brucella melitensis Reservoir Cells during the Chronic Phase of Infection in Susceptible Mice. PLoS One 10:e0137835 (2015). PubMed: 26376185
