APC Anti-F4/80 antibody [BM8.1] (ab210250)
![Flow Cytometry - APC Anti-F4/80 antibody [BM8.1] (ab210250)](https://www.abcam.com/ps/products/210/ab210250/Images/ab210250-256787-anti-f4-80-antibody-bm81-allophycocyanin-flow-cytometry.jpg "Flow Cytometry - APC Anti-F4/80 antibody [BM8.1] (ab210250)")
Key features and details
- APC Rat monoclonal [BM8.1] to F4/80
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG2a
Overview
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Product name
APC Anti-F4/80 antibody [BM8.1]
See all F4/80 primary antibodies -
Description
APC Rat monoclonal [BM8.1] to F4/80 -
Host species
Rat -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Mouse C57Bl/6 bone marrow cells
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General notes
ab210250 is widely used together with antibodies to CD115, CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.
The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
ab210250 was purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
BM8.1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
Tissue specificity
Expression is restricted to eosinophils. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 13733 Mouse
- SwissProt: Q61549 Mouse
- Unigene: 2254 Mouse
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Alternative names
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
Images
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Flow Cytometry - APC Anti-F4/80 antibody [BM8.1] (ab210250)
Flow cytometric analysis of F4/80 in mouse C57Bl/6 bone marrow cells using FITC Anti-Mouse CD11b and 0.5 μg ab210250 (right panel), or 0.5 μg Allophycocyanin Rat IgG2a isotype control (left panel).
Datasheets and documents
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Datasheet download
References (1)
ab210250 has been referenced in 1 publication.
- Hanot Mambres D et al. In Situ Characterization of Splenic Brucella melitensis Reservoir Cells during the Chronic Phase of Infection in Susceptible Mice. PLoS One 10:e0137835 (2015). PubMed: 26376185
