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  1. Link

    apc-f480-antibody-bm81-ab210250.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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APC Anti-F4/80 antibody [BM8.1] (ab210250)

  • Datasheet
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Flow Cytometry - APC Anti-F4/80 antibody [BM8.1] (ab210250)

    Key features and details

    • APC Rat monoclonal [BM8.1] to F4/80
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: APC. Ex: 645nm, Em: 660nm
    • Isotype: IgG2a

    You may also be interested in

    Protein
    Product image
    Recombinant Human EMR1/ADGRE1 protein (ab131740)

    View more associated products

    Overview

    • Product name

      APC Anti-F4/80 antibody [BM8.1]
      See all F4/80 primary antibodies
    • Description

      APC Rat monoclonal [BM8.1] to F4/80
    • Host species

      Rat
    • Conjugation

      APC. Ex: 645nm, Em: 660nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • Mouse C57Bl/6 bone marrow cells
    • General notes

      ab210250 is widely used together with antibodies to CD115, CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

      The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
    • Concentration information loading...
    • Purity

      Affinity purified
    • Purification notes

      ab210250 was purified from tissue culture supernatant.
    • Clonality

      Monoclonal
    • Clone number

      BM8.1
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Tags & Cell Markers
      • Cell Type Markers
      • Other Cell Types

    Associated products

    • Alternative Versions

      • PerCP/Cy5.5® Anti-F4/80 antibody [BM8.1] (ab210252)
      • PE/Cy7® Anti-F4/80 antibody [BM8.1] (ab218761)
      • violetFluor™ 450 Anti-F4/80 antibody [BM8.1] (ab242258)
      • redFluor™ 710 Anti-F4/80 antibody [BM8.1] (ab242260)
    • Recombinant Protein

      • Recombinant Human EMR1/ADGRE1 protein (ab131740)

    Applications

    Our Abpromise guarantee covers the use of ab210250 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.

    Target

    • Function

      Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
    • Tissue specificity

      Expression is restricted to eosinophils.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
      Contains 6 EGF-like domains.
      Contains 1 GPS domain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession Q14246 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 13733 Mouse
      • SwissProt: Q61549 Mouse
      • Unigene: 2254 Mouse
      • Alternative names

        • ADGRE1 antibody
        • Adhesion G protein coupled receptor E1 antibody
        • Adhesion G protein-coupled receptor E1 antibody
        • AGRE1_HUMAN antibody
        • Cell surface glycoprotein EMR1 antibody
        • Cell surface glycoprotein F4/80 antibody
        • DD7A5 7 antibody
        • Egf like module containing mucin like hormone receptor like 1 antibody
        • Egf like module containing mucin like hormone receptor like sequence 1 antibody
        • EGF like module receptor 1 antibody
        • EGF TM7 antibody
        • EGF-like module receptor 1 antibody
        • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
        • EGFTM7 antibody
        • EMR 1 antibody
        • EMR1 antibody
        • EMR1 hormone receptor antibody
        • Gpf480 antibody
        • Ly71 antibody
        • Lymphocyte antigen 71 antibody
        • TM7LN3 antibody
        see all

      Images

      • Flow Cytometry - APC Anti-F4/80 antibody [BM8.1] (ab210250)
        Flow Cytometry - APC Anti-F4/80 antibody [BM8.1] (ab210250)

        Flow cytometric analysis of F4/80 in mouse C57Bl/6 bone marrow cells using FITC Anti-Mouse CD11b and 0.5 μg ab210250 (right panel), or 0.5 μg Allophycocyanin Rat IgG2a isotype control (left panel).

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab210250? Please let us know so that we can cite the reference in this datasheet.

      ab210250 has been referenced in 1 publication.

      • Hanot Mambres D  et al. In Situ Characterization of Splenic Brucella melitensis Reservoir Cells during the Chronic Phase of Infection in Susceptible Mice. PLoS One 10:e0137835 (2015). PubMed: 26376185

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab210250.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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