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  1. Link

    apc-f480-antibody-epr20548-ab237333.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Recombinant APC Anti-F4/80 antibody [EPR20548] (ab237333)

  • Datasheet
  • Certificate of Compliance
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Flow Cytometry - APC Anti-F4/80 antibody [EPR20548] (ab237333)
  • APC Anti-F4/80 antibody [EPR20548] (ab237333)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • APC Rabbit monoclonal [EPR20548] to F4/80
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Conjugation: APC. Ex: 645nm, Em: 660nm

You may also be interested in

Protein
Product image
Recombinant Human EMR1/ADGRE1 protein (ab131740)

View more associated products

Overview

  • Product name

    APC Anti-F4/80 antibody [EPR20548]
    See all F4/80 primary antibodies
  • Description

    APC Rabbit monoclonal [EPR20548] to F4/80
  • Host species

    Rabbit
  • Conjugation

    APC. Ex: 645nm, Em: 660nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse F4/80 aa 350-650. The exact sequence is proprietary.
    Database link: Q61549

  • Positive control

    • Flow Cyt: C57/BL6 splenocytes.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20548
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types

Associated products

  • Alternative Versions

    • Anti-F4/80 antibody [EPR20548] (ab213200)
    • Anti-F4/80 antibody [EPR20548] - BSA and Azide free (ab234530)
    • Alexa Fluor® 488 Anti-F4/80 antibody [EPR20548] (ab237331)
    • Alexa Fluor® 647 Anti-F4/80 antibody [EPR20548] (ab237332)
    • PE Anti-F4/80 antibody [EPR20548] (ab237335)
  • Recombinant Protein

    • Recombinant Human EMR1/ADGRE1 protein (ab131740)
  • Related Products

    • APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

Applications

Our Abpromise guarantee covers the use of ab237333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

Target

  • Function

    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificity

    Expression is restricted to eosinophils.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q14246 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 13733 Mouse
    • SwissProt: Q61549 Mouse
    • Unigene: 2254 Mouse
    • Alternative names

      • ADGRE1 antibody
      • Adhesion G protein coupled receptor E1 antibody
      • Adhesion G protein-coupled receptor E1 antibody
      • AGRE1_HUMAN antibody
      • Cell surface glycoprotein EMR1 antibody
      • Cell surface glycoprotein F4/80 antibody
      • DD7A5 7 antibody
      • Egf like module containing mucin like hormone receptor like 1 antibody
      • Egf like module containing mucin like hormone receptor like sequence 1 antibody
      • EGF like module receptor 1 antibody
      • EGF TM7 antibody
      • EGF-like module receptor 1 antibody
      • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
      • EGFTM7 antibody
      • EMR 1 antibody
      • EMR1 antibody
      • EMR1 hormone receptor antibody
      • Gpf480 antibody
      • Ly71 antibody
      • Lymphocyte antigen 71 antibody
      • TM7LN3 antibody
      see all

    Images

    • Flow Cytometry - APC Anti-F4/80 antibody [EPR20548] (ab237333)
      Flow Cytometry - APC Anti-F4/80 antibody [EPR20548] (ab237333)

      C57BL/6 mouse splenocytes stained with ab237333 (right) or Rabbit IgG (monoclonal) Allophycocyanin (ab232814) (left). C57BL/6 mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10µg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab237333) or Rabbit IgG (monoclonal) Allophycocyanin (ab232814) (1x 106 in 100µl at 1µg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with CD11b. Acquisition of >30,000 events were collected using a 40 mW Red laser (640 nm) and 660/20 bandpass filter. Events were gated on viable CD19 and CD3 negative lymphocytes.

    • APC Anti-F4/80 antibody [EPR20548] (ab237333)
      APC Anti-F4/80 antibody [EPR20548] (ab237333)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab237333? Please let us know so that we can cite the reference in this datasheet.

    ab237333 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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