Recombinant APC Anti-F4/80 antibody [EPR20548] (ab237333)
![Flow Cytometry - APC Anti-F4/80 antibody [EPR20548] (ab237333)](https://www.abcam.com/ps/products/237/ab237333/Images/ab237333-351182-antiF480ab237333Flow-CytometryC57BL6-MouseSpleen.jpg "Flow Cytometry - APC Anti-F4/80 antibody [EPR20548] (ab237333)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EPR20548] to F4/80
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
Overview
-
Product name
APC Anti-F4/80 antibody [EPR20548]
See all F4/80 primary antibodies -
Description
APC Rabbit monoclonal [EPR20548] to F4/80 -
Host species
Rabbit -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide within Mouse F4/80 aa 350-650. The exact sequence is proprietary.
Database link: Q61549 -
Positive control
- Flow Cyt: C57/BL6 splenocytes.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20548 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab237333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. |
Target
-
Function
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
Tissue specificity
Expression is restricted to eosinophils. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 13733 Mouse
- SwissProt: Q61549 Mouse
- Unigene: 2254 Mouse
-
Alternative names
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
Images
-
Flow Cytometry - APC Anti-F4/80 antibody [EPR20548] (ab237333)
C57BL/6 mouse splenocytes stained with ab237333 (right) or Rabbit IgG (monoclonal) Allophycocyanin (ab232814) (left). C57BL/6 mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10µg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab237333) or Rabbit IgG (monoclonal) Allophycocyanin (ab232814) (1x 106 in 100µl at 1µg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with CD11b. Acquisition of >30,000 events were collected using a 40 mW Red laser (640 nm) and 660/20 bandpass filter. Events were gated on viable CD19 and CD3 negative lymphocytes.
-
![APC Anti-F4/80 antibody [EPR20548] (ab237333)](https://www.abcam.com/ps/products/237/ab237333/Images/ab237333-2-benefits-of-recombinant-antibodies.png)
APC Anti-F4/80 antibody [EPR20548] (ab237333)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab237333 has not yet been referenced specifically in any publications.
