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  1. Link

    apc-f480-antibody-epr20548-ab237333.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Recombinant APC Anti-F4/80 antibody [EPR20548] (ab237333)

  • Datasheet
  • Certificate of Compliance
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Flow Cytometry - APC Anti-F4/80 antibody [EPR20548] (ab237333)
  • APC Anti-F4/80 antibody [EPR20548] (ab237333)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • APC Rabbit monoclonal [EPR20548] to F4/80
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Conjugation: APC. Ex: 645nm, Em: 660nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE Unconjugated

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Anti-F4/80 antibody [EPR20548] - BSA and Azide free (ab234530)

View more associated products

Overview

  • Product name

    APC Anti-F4/80 antibody [EPR20548]
    See all F4/80 primary antibodies
  • Description

    APC Rabbit monoclonal [EPR20548] to F4/80
  • Host species

    Rabbit
  • Conjugation

    APC. Ex: 645nm, Em: 660nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: C57/BL6 splenocytes.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20548
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types

Associated products

  • Alternative Versions

    • Anti-F4/80 antibody [EPR20548] (ab213200)
    • Anti-F4/80 antibody [EPR20548] - BSA and Azide free (ab234530)
    • Alexa Fluor® 488 Anti-F4/80 antibody [EPR20548] (ab237331)
    • Alexa Fluor® 647 Anti-F4/80 antibody [EPR20548] (ab237332)
    • PE Anti-F4/80 antibody [EPR20548] (ab237335)
  • Recombinant Protein

    • Recombinant Human EMR1/ADGRE1 protein (ab131740)
  • Related Products

    • APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab237333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/500.
Notes
Flow Cyt
1/500.

Target

  • Function

    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificity

    Expression is restricted to eosinophils.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q14246 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 13733 Mouse
    • SwissProt: Q61549 Mouse
    • Unigene: 2254 Mouse
    • Alternative names

      • ADGRE1 antibody
      • Adhesion G protein coupled receptor E1 antibody
      • Adhesion G protein-coupled receptor E1 antibody
      • AGRE1_HUMAN antibody
      • Cell surface glycoprotein EMR1 antibody
      • Cell surface glycoprotein F4/80 antibody
      • DD7A5 7 antibody
      • Egf like module containing mucin like hormone receptor like 1 antibody
      • Egf like module containing mucin like hormone receptor like sequence 1 antibody
      • EGF like module receptor 1 antibody
      • EGF TM7 antibody
      • EGF-like module receptor 1 antibody
      • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
      • EGFTM7 antibody
      • EMR 1 antibody
      • EMR1 antibody
      • EMR1 hormone receptor antibody
      • Gpf480 antibody
      • Ly71 antibody
      • Lymphocyte antigen 71 antibody
      • TM7LN3 antibody
      see all

    Images

    • Flow Cytometry - APC Anti-F4/80 antibody [EPR20548] (ab237333)
      Flow Cytometry - APC Anti-F4/80 antibody [EPR20548] (ab237333)

      C57BL/6 mouse splenocytes stained with ab237333 (right) or Rabbit IgG (monoclonal) Allophycocyanin (ab232814) (left). C57BL/6 mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10µg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab237333) or Rabbit IgG (monoclonal) Allophycocyanin (ab232814) (1x 106 in 100µl at 1µg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with CD11b. Acquisition of >30,000 events were collected using a 40 mW Red laser (640 nm) and 660/20 bandpass filter. Events were gated on viable CD19 and CD3 negative lymphocytes.

    • APC Anti-F4/80 antibody [EPR20548] (ab237333)
      APC Anti-F4/80 antibody [EPR20548] (ab237333)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab237333? Please let us know so that we can cite the reference in this datasheet.

    ab237333 has not yet been referenced specifically in any publications.

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