APC Anti-GCSF Receptor antibody [06] (ab275590)
![Flow Cytometry - APC Anti-GCSF Receptor antibody [06] (ab275590)](https://www.abcam.com/ps/products/275/ab275590/Images/ab275590-411058-anti-gcsf-receptor-antibody-06-apc-flow-cytometry-granulocytes-human.jpg "Flow Cytometry - APC Anti-GCSF Receptor antibody [06] (ab275590)")
Key features and details
- APC Mouse monoclonal [06] to GCSF Receptor
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Overview
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Product name
APC Anti-GCSF Receptor antibody [06]
See all GCSF Receptor primary antibodies -
Description
APC Mouse monoclonal [06] to GCSF Receptor -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human GCSF Receptor aa 1-650 (extracellular). NP_000751.1. Extracellular domain with five aminio acids at the C-terminus.
Database link: Q99062 -
Positive control
- Flow Cyt: Human whole blood granulocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
06 -
Isotype
IgG1 -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
Use 10 μl/test. |
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. Use 10 μl/test. |
Target
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Function
Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface. -
Tissue specificity
One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta. -
Involvement in disease
Hereditary neutrophilia -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1441 Human
- Omim: 138971 Human
- SwissProt: Q99062 Human
- Unigene: 524517 Human
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Alternative names
- CD 114 antibody
- CD114 antibody
- CD114 antigen antibody
see all
Images
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Flow Cytometry - APC Anti-GCSF Receptor antibody [06] (ab275590)
Flow cytometric analysis of human whole blood granulocytes labeling GCSF Receptor with ab275590 at 10 μL per test (Black) compared to an isotype control (Grey). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable granulocytes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab275590 has not yet been referenced specifically in any publications.
