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  1. Link

    apc-icam1-antibody-yn1174-ab87026.pdf

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Immunology Cell Type Markers CD Adhesion
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APC Anti-ICAM1 antibody [YN1/1.7.4] (ab87026)

  • Datasheet
  • SDS
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Flow Cytometry - APC Anti-ICAM1 antibody [YN1/1.7.4] (ab87026)
  • Flow Cytometry - APC Anti-ICAM1 antibody [YN1/1.7.4] (ab87026)

Key features and details

  • APC Rat monoclonal [YN1/1.7.4] to ICAM1
  • Reacts with: Mouse
  • Conjugation: APC. Ex: 645nm, Em: 660nm
  • Isotype: IgG2b

You may also be interested in

Protein
Recombinant Human ICAM1 protein (ab151393)

View more associated products

Overview

  • Product name

    APC Anti-ICAM1 antibody [YN1/1.7.4]
    See all ICAM1 primary antibodies
  • Description

    APC Rat monoclonal [YN1/1.7.4] to ICAM1
  • Host species

    Rat
  • Conjugation

    APC. Ex: 645nm, Em: 660nm
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • Flow Cyt: C57BL/6 mouse splenocytes.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.3
    Preservative: 0.1% Sodium azide
    Constituent: PBS

    Also contains a stabilizing agent.
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    YN1/1.7.4
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell adhesion molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • Anti-ICAM1 antibody [YN1/1.7.4] (ab119871)
    • FITC Anti-ICAM1 antibody [YN1/1.7.4] (ab24869)
    • Biotin Anti-ICAM1 antibody [YN1/1.7.4] (ab25007)
    • Anti-ICAM1 antibody [YN1/1.7.4] (ab25375)
    • PE Anti-ICAM1 antibody [YN1/1.7.4] (ab25553)
  • Isotype control

    • APC Rat IgG2b, kappa monoclonal [KLH/G2b-1-2] - Isotype control (ab154434)
  • Recombinant Protein

    • Recombinant Human ICAM1 protein (ab151393)

Applications

  • Application notes
    Flow Cyt: Use 0.2µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    Inhib: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 58 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications

      Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P05362 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 15894 Mouse
      • SwissProt: P13597 Mouse
      • Unigene: 435508 Mouse
      • Alternative names

        • Antigen identified by monoclonal antibody BB2 antibody
        • BB 2 antibody
        • BB2 antibody
        • CD 54 antibody
        • CD_antigen=CD54 antibody
        • CD54 antibody
        • Cell surface glycoprotein P3.58 antibody
        • Human rhinovirus receptor antibody
        • ICAM 1 antibody
        • ICAM-1 antibody
        • ICAM1 antibody
        • ICAM1_HUMAN antibody
        • intercellular adhesion molecule 1 (CD54) antibody
        • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
        • Intercellular adhesion molecule 1 antibody
        • Major group rhinovirus receptor antibody
        • MALA 2 antibody
        • MALA2 antibody
        • MyD 10 antibody
        • MyD10 antibody
        • P3.58 antibody
        • Surface antigen of activated B cells antibody
        • Surface antigen of activated B cells, BB2 antibody
        see all

      Images

      • Flow Cytometry - APC Anti-ICAM1 antibody [YN1/1.7.4] (ab87026)
        Flow Cytometry - APC Anti-ICAM1 antibody [YN1/1.7.4] (ab87026)

        Flow Cytometry analysis of C57BL/6 mouse splenocytes stained for ICAM1 using ab87026 (0.1 µg/106 cells) and a Rat Anti-Mouse NK1.1-FITC.

      • Flow Cytometry - APC Anti-ICAM1 antibody [YN1/1.7.4] (ab87026)
        Flow Cytometry - APC Anti-ICAM1 antibody [YN1/1.7.4] (ab87026)

        Flow Cytometry analysis of C57BL/6 mouse splenocytes stained for ICAM1 using ab87026 (0.1 µg/106 cells) and a Rat Anti-Mouse CD54-APC.

      Protocols

      • Flow cytometry protocols
      • Immunoprecipitation protocols
      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab87026? Please let us know so that we can cite the reference in this datasheet.

      ab87026 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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