APC Anti-Integrin alpha 2 antibody [DX5] (ab41460)
![Flow Cytometry - APC Anti-Integrin alpha 2 antibody [DX5] (ab41460)](https://www.abcam.com/ps/products/41/ab41460/Images/ab41460-1-anti-integrin-alpha-2-antibody-dx5-apc-flow-cytometry-splenocytes-mouse.jpg "Flow Cytometry - APC Anti-Integrin alpha 2 antibody [DX5] (ab41460)")
Key features and details
- APC Rat monoclonal [DX5] to Integrin alpha 2
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgM
Overview
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Product name
APC Anti-Integrin alpha 2 antibody [DX5]
See all Integrin alpha 2 primary antibodies -
Description
APC Rat monoclonal [DX5] to Integrin alpha 2 -
Host species
Rat -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse Integrin alpha 2. NK cells isolated from C57BL/6 mice
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Positive control
- Flow Cyt: C57BL/6 mouse splenocytes.
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General notes
This monoclonal antibody, clone DX5, reacts with CD49b, also known as very late antigen-2 (VLA-2) and Integrin alpha 2. The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes.2 NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Also contains stabilising agent. -
Concentration information loading... -
Purity
Purified IgM -
Primary antibody notes
This monoclonal antibody, clone DX5, reacts with CD49b, also known as very late antigen-2 (VLA-2) and Integrin alpha 2. The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes.2 NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity. -
Clonality
Monoclonal -
Clone number
DX5 -
Myeloma
Sp2/0 -
Isotype
IgM -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16398 Mouse
- SwissProt: Q62469 Mouse
- Unigene: 5007 Mouse
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Alternative names
- BR antibody
- CD 49b antibody
- CD49 antigen like family member B antibody
see all
Images
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Flow Cytometry - APC Anti-Integrin alpha 2 antibody [DX5] (ab41460)
Flow Cytometry analysis of C57BL/6 mouse splenocytes stained for Integrin alpha 2 using ab41460 (0.1 µg/106 cells) and a Mouse Anti-Mouse NK1.1-PE.
Datasheets and documents
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Datasheet download
References (2)
ab41460 has been referenced in 2 publications.
- Arase H et al. Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2). J Immunol 167:1141-4 (2001). PubMed: 11466327
- Moore TA et al. Inhibition of gamma delta T cell development and early thymocyte maturation in IL-7 -/- mice. J Immunol 157:2366-73 (1996). PubMed: 8805634
