APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (ab93539)

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Flow Cytometry - APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (ab93539)

    Key features and details

    • APC Rat monoclonal [DATK32 (DATK-32)] to Integrin alpha 4+beta 7
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: APC. Ex: 645nm, Em: 660nm
    • Isotype: IgG2a

    Overview

    • Product name

      APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)]
      See all Integrin alpha 4+beta 7 primary antibodies

    • Description

      APC Rat monoclonal [DATK32 (DATK-32)] to Integrin alpha 4+beta 7

    • Host species

      Rat

    • Conjugation

      APC. Ex: 645nm, Em: 660nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      Tissue/ cell preparation: AKR/Cum Mouse spontaneous T lymphoma cell line TK1.

    • Positive control

      • Flow Cyt: Mouse spleen cells and C57Bl/6 bone marrow cells.

    • General notes

      Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block Integrin alpha 4 beta 7 mediated cell adhesion.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Associated products

      Applications

      Our Abpromise guarantee covers the use of ab93539 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      Flow Cyt Use at an assay dependent concentration.

      Target

      • Relevance

        Integrin alpha 4 beta 7, also known as, the lymphocyte Peyer's patch adhesion molecule (LPAM1) is a member of the integrin family of cell surface receptors. It is expressed primarily on mucosal lymphocytes, but is also present on NK cells and eosinophils. The alpha 4 beta 7 heterodimer mediates the binding of lymphocytes to its ligand, mucosal vascular addressin (MAdCAM1) on the high endothelial venules, thereby directing the homing of lymphocytes into Peyer's patches and intestinal lamina propria.

      • Cellular localization

        Membrane; Single pass type I membrane protein.

      • Database links

        • Alternative names

          • Antigen CD49D antibody
          • CD49d antibody
          • IA4 antibody
          • Integrin alpha 4 subunit antibody
          • Integrin alpha IV antibody
          • Integrin beta 7 antibody
          • Integrin beta 7 subunit antibody
          • ITGA4 antibody
          • ITGB7 antibody
          • LPAM1 antibody
          see all

        Images

        • Flow Cytometry - APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (ab93539)
          Flow Cytometry - APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (ab93539)

          C57Bl/6 bone marrow cells stained with FITC-conjugated anti-mouse CD45 (B220) and 0.25µg ab93539 (right) or 0.25µg APC-conjugated rat IgG2a isotype control (left).

        References (3)

        Publishing research using ab93539? Please let us know so that we can cite the reference in this datasheet.

        ab93539 has been referenced in 3 publications.

        • Andrew DP  et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol 153:3847-61 (1994). Flow Cyt . PubMed: 7523506
        • Hamann A  et al. Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo. J Immunol 152:3282-93 (1994). Flow Cyt . PubMed: 7511642
        • Berlin C  et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74:185-95 (1993). Flow Cyt . PubMed: 7687523

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