For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    apc-integrin-alpha-4beta-7-antibody-datk32-datk-32-ab93539.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
Share by email

APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (ab93539)

  • Datasheet
Submit a review Submit a question References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Flow Cytometry - APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (ab93539)

    Key features and details

    • APC Rat monoclonal [DATK32 (DATK-32)] to Integrin alpha 4+beta 7
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: APC. Ex: 645nm, Em: 660nm
    • Isotype: IgG2a

    You may also be interested in

    Conjugation
    Product image
    APC Conjugation Kit - Lightning-Link® (ab201807)
    Primary
    Product image
    Anti-Integrin alpha 4+beta 7 antibody [RM0059-2G18] (ab73261)

    View more associated products

    Overview

    • Product name

      APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)]
      See all Integrin alpha 4+beta 7 primary antibodies
    • Description

      APC Rat monoclonal [DATK32 (DATK-32)] to Integrin alpha 4+beta 7
    • Host species

      Rat
    • Conjugation

      APC. Ex: 645nm, Em: 660nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Tissue/ cell preparation: AKR/Cum Mouse spontaneous T lymphoma cell line TK1.

    • Positive control

      • Flow Cyt: Mouse spleen cells and C57Bl/6 bone marrow cells.
    • General notes

      Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block Integrin alpha 4 beta 7 mediated cell adhesion.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.88% Sodium chloride, 0.1% Gelatin
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Primary antibody notes

      Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block Integrin alpha 4 beta 7 mediated cell adhesion.
    • Clonality

      Monoclonal
    • Clone number

      DATK32 (DATK-32)
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Integrins
      • Alpha
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Integrins
      • Beta
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Immunology
      • Immune System Diseases
      • Antiviral Signaling
      • HIV-related

    Associated products

      Applications

      Our Abpromise guarantee covers the use of ab93539 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      Flow Cyt Use at an assay dependent concentration.

      Target

      • Relevance

        Integrin alpha 4 beta 7, also known as, the lymphocyte Peyer's patch adhesion molecule (LPAM1) is a member of the integrin family of cell surface receptors. It is expressed primarily on mucosal lymphocytes, but is also present on NK cells and eosinophils. The alpha 4 beta 7 heterodimer mediates the binding of lymphocytes to its ligand, mucosal vascular addressin (MAdCAM1) on the high endothelial venules, thereby directing the homing of lymphocytes into Peyer's patches and intestinal lamina propria.
      • Cellular localization

        Membrane; Single pass type I membrane protein.
      • Database links

        • Entrez Gene: 16401 Mouse
        • Entrez Gene: 16421 Mouse
        • SwissProt: P26011 Mouse
        • SwissProt: Q00651 Mouse
        • Alternative names

          • Antigen CD49D antibody
          • CD49d antibody
          • IA4 antibody
          • Integrin alpha 4 subunit antibody
          • Integrin alpha IV antibody
          • Integrin beta 7 antibody
          • Integrin beta 7 subunit antibody
          • ITGA4 antibody
          • ITGB7 antibody
          • LPAM1 antibody
          see all

        Images

        • Flow Cytometry - APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (ab93539)
          Flow Cytometry - APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (ab93539)

          C57Bl/6 bone marrow cells stained with FITC-conjugated anti-mouse CD45 (B220) and 0.25µg ab93539 (right) or 0.25µg APC-conjugated rat IgG2a isotype control (left).

        Protocols

        • Flow cytometry protocols

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
      • References (3)

        Publishing research using ab93539? Please let us know so that we can cite the reference in this datasheet.

        ab93539 has been referenced in 3 publications.

        • Andrew DP  et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol 153:3847-61 (1994). Flow Cyt . PubMed: 7523506
        • Hamann A  et al. Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo. J Immunol 152:3282-93 (1994). Flow Cyt . PubMed: 7511642
        • Berlin C  et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74:185-95 (1993). Flow Cyt . PubMed: 7687523

        Customer reviews and Q&As

        Show All Reviews Q&A
        Submit a review Submit a question

        There are currently no Customer reviews or Questions for ab93539.
        Please use the links above to contact us or submit feedback about this product.

        Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
        For licensing inquiries, please contact partnerships@abcam.com

        Get resources and offers direct to your inbox Sign up
        A-Z by research area
        • Cancer
        • Cardiovascular
        • Cell biology
        • Developmental biology
        • Epigenetics & Nuclear signaling
        • Immunology
        • Metabolism
        • Microbiology
        • Neuroscience
        • Signal transduction
        • Stem cells
        A-Z by product type
        • Primary antibodies
        • Secondary antibodies
        • Biochemicals
        • Isotype controls
        • Flow cytometry multi-color selector
        • Kits
        • Loading controls
        • Lysates
        • Peptides
        • Proteins
        • Slides
        • Tags and cell markers
        • Tools & Reagents
        Help & support
        • Support
        • Make an Inquiry
        • Protocols & troubleshooting
        • Placing an order
        • RabMAb products
        • Biochemical product FAQs
        • Training
        • Browse by Target
        Company
        • Corporate site
        • Investor relations
        • Company news
        • Careers
        • About us
        • Blog
        Events
        • Tradeshows
        • Conferences
        International websites
        • abcam.cn
        • abcam.co.jp

        Join with us

        • LinkedIn
        • facebook
        • Twitter
        • YouTube
        • Terms of sale
        • Website terms of use
        • Cookie policy
        • Privacy policy
        • Legal
        • Modern slavery statement
        © 1998-2021 Abcam plc. All rights reserved.