APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (ab93539)
![Flow Cytometry - APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (ab93539)](https://www.abcam.com/ps/products/93/ab93539/Images/ab93539-277182-ab93539fc.jpg "Flow Cytometry - APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (ab93539)")
Key features and details
- APC Rat monoclonal [DATK32 (DATK-32)] to Integrin alpha 4+beta 7
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG2a
Overview
-
Product name
APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)]
See all Integrin alpha 4+beta 7 primary antibodies -
Description
APC Rat monoclonal [DATK32 (DATK-32)] to Integrin alpha 4+beta 7 -
Host species
Rat -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse Integrin alpha 4+beta 7. TK-1 cell line
-
Positive control
- Flow Cyt: Mouse spleen cells and C57Bl/6 bone marrow cells.
-
General notes
Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block Integrin alpha 4 beta 7 mediated cell adhesion.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.88% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block Integrin alpha 4 beta 7 mediated cell adhesion. -
Clonality
Monoclonal -
Clone number
DATK32 (DATK-32) -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
Target
-
Relevance
Integrin alpha 4 beta 7, also known as, the lymphocyte Peyer's patch adhesion molecule (LPAM1) is a member of the integrin family of cell surface receptors. It is expressed primarily on mucosal lymphocytes, but is also present on NK cells and eosinophils. The alpha 4 beta 7 heterodimer mediates the binding of lymphocytes to its ligand, mucosal vascular addressin (MAdCAM1) on the high endothelial venules, thereby directing the homing of lymphocytes into Peyer's patches and intestinal lamina propria. -
Cellular localization
Membrane; Single pass type I membrane protein. -
Database links
- Entrez Gene: 16401 Mouse
- Entrez Gene: 16421 Mouse
- SwissProt: P26011 Mouse
- SwissProt: Q00651 Mouse
-
Alternative names
- Antigen CD49D antibody
- CD49d antibody
- IA4 antibody
see all
Images
-
Flow Cytometry - APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (ab93539)
C57Bl/6 bone marrow cells stained with FITC-conjugated anti-mouse CD45 (B220) and 0.25µg ab93539 (right) or 0.25µg APC-conjugated rat IgG2a isotype control (left).
Datasheets and documents
-
Datasheet download
References (3)
ab93539 has been referenced in 3 publications.
- Andrew DP et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol 153:3847-61 (1994). Flow Cyt . PubMed: 7523506
- Hamann A et al. Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo. J Immunol 152:3282-93 (1994). Flow Cyt . PubMed: 7511642
- Berlin C et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74:185-95 (1993). Flow Cyt . PubMed: 7687523
