APC Anti-Interferon gamma antibody [4S.B3] (ab188415)

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Flow Cytometry (Intracellular) - APC Anti-Interferon gamma antibody [4S.B3] (ab188415)

    Key features and details

    • APC Mouse monoclonal [4S.B3] to Interferon gamma
    • Suitable for: Flow Cyt (Intra)
    • Reacts with: Human
    • Conjugation: APC. Ex: 645nm, Em: 660nm
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Biotin PE PE/Cy7® PerCP/Cy5.5® Unconjugated

    Overview

    • Product name

      APC Anti-Interferon gamma antibody [4S.B3]
      See all Interferon gamma primary antibodies

    • Description

      APC Mouse monoclonal [4S.B3] to Interferon gamma

    • Host species

      Mouse

    • Conjugation

      APC. Ex: 645nm, Em: 660nm

    • Specificity

      Binds both glycosylated and non-glycosylated protein.

    • Tested applications

      Suitable for: Flow Cyt (Intra)more details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Full length native protein (purified) corresponding to Human Interferon gamma aa 1-166. Interferon gamma derived from human leukocytes.
      Database link: P01579

    • Positive control

      • Flow Cyt (Intra): PHA activated human PBMC

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab188415 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt (Intra)
    Use 10µg for 106 cells.

    or 100 µl of whole blood
    Notes
    Flow Cyt (Intra)
    Use 10µg for 106 cells.

    or 100 µl of whole blood

    Target

    • Function

      Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

    • Tissue specificity

      Released primarily from activated T lymphocytes.

    • Involvement in disease

      In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

    • Sequence similarities

      Belongs to the type II (or gamma) interferon family.

    • Post-translational
      modifications

      Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • IF 1 antibody
        • IFG antibody
        • IFI antibody
        • ifn g antibody
        • IFN gamma antibody
        • IFN immune antibody
        • IFN, immune antibody
        • IFN-gamma antibody
        • IFNG antibody
        • IFNG_HUMAN antibody
        • Immune interferon antibody
        • Interferon gamma antibody
        • Type II Interferon antibody
        see all

      Images

      • Flow Cytometry (Intracellular) - APC Anti-Interferon gamma antibody [4S.B3] (ab188415)
        Flow Cytometry (Intracellular) - APC Anti-Interferon gamma antibody [4S.B3] (ab188415)
        Flow Cytometry analysis of PHA-activated human PBMC cells labeling Interferon gamma with Anti-Interferon gamma antibody [4S.B3] (Allophycocyanin) (ab188415).

      References (1)

      Publishing research using ab188415? Please let us know so that we can cite the reference in this datasheet.

      ab188415 has been referenced in 1 publication.

      • Li X  et al. Peripheral blood invariant natural killer T cells of pig-tailed macaques. PLoS One 7:e48166 (2012). PubMed: 23110202

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