Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EPR23246-275] to Junctional Adhesion Molecule 1/JAM-A
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
Related conjugates and formulations
Product nameAPC Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275]
See all Junctional Adhesion Molecule 1/JAM-A primary antibodies
DescriptionAPC Rabbit monoclonal [EPR23246-275] to Junctional Adhesion Molecule 1/JAM-A
ConjugationAPC. Ex: 645nm, Em: 660nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
- Flow cyt: bEnd.3 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] (ab270446)
- Alexa Fluor® 488 Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] (ab275179)
- Alexa Fluor® 647 Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] (ab275180)
- PE Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] (ab275182)
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionSeems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.
Sequence similaritiesBelongs to the immunoglobulin superfamily.
Contains 2 Ig-like V-type (immunoglobulin-like) domains.
Cellular localizationCell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
- Information by UniProt
- CD 321 antibody
- CD321 antibody
- CD321 antigen antibody
Flow cytometry overlay histogram showing (left) Bend.3 positive cells and right negative Neuro2a cells stained with ab275181 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab275181) (1x 106 in 100μl at 5μg/ml (1/100)) for 30 min on ice.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab275181 has not yet been referenced specifically in any publications.