APC Anti-Ly6c antibody [HK1.4] (ab93550)

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Flow Cytometry - APC Anti-Ly6c antibody [HK1.4] (ab93550)

    Key features and details

    • APC Rat monoclonal [HK1.4] to Ly6c
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: APC. Ex: 645nm, Em: 660nm
    • Isotype: IgG2c

    Overview

    • Product name

      APC Anti-Ly6c antibody [HK1.4]
      See all Ly6c primary antibodies

    • Description

      APC Rat monoclonal [HK1.4] to Ly6c

    • Host species

      Rat

    • Conjugation

      APC. Ex: 645nm, Em: 660nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • BALB/c splenocytes

    • General notes


      In vitro addition of clone HK1.4 monoclonal antibody can increase proliferation and stimulate cytokine release.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab93550 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 0.125µg for 105-8 cells.

    0.125µg for 105 to 108 cells in a final volume of 100 µl.

     

     

     

     

    ab154435 - Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody.

     

    Target

    • Relevance

      Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.

    • Cellular localization

      Cell membrane; Lipid-anchor, GPI-anchor.

    • Database links

      • Alternative names

        • Ly 6c antibody
        • Ly6c protein antibody
        • Ly6c1 antibody
        • Ly6c2 antibody
        • Lymphocyte antigen 6 complex antibody
        • Lymphocyte antigen 6 complex locus C antibody
        • Lymphocyte antigen 6C antibody
        • Lymphocyte antigen 6C1 antibody
        • Lymphocyte antigen 6C2 antibody
        • Lymphocyte antigen Ly 6C antibody
        see all

      Images

      • Flow Cytometry - APC Anti-Ly6c antibody [HK1.4] (ab93550)
        Flow Cytometry - APC Anti-Ly6c antibody [HK1.4] (ab93550)
        Staining of BALB/c splenocytes with FITC anti-mouse CD8 and 0.06 µg of ab93550. Total viable cells were used for analysis.

      References (3)

      Publishing research using ab93550? Please let us know so that we can cite the reference in this datasheet.

      ab93550 has been referenced in 3 publications.

      • Wu M  et al. MR imaging tracking of inflammation-activatable engineered neutrophils for targeted therapy of surgically treated glioma. Nat Commun 9:4777 (2018). PubMed: 30429468
      • Pflugh DL  et al. Ly-6 superfamily members Ly-6A/E, Ly-6C, and Ly-6I recognize two potential ligands expressed by B lymphocytes. J Immunol 169:5130-6 (2002). PubMed: 12391229
      • Havran WL  et al. Characterization of an anti-Ly-6 monoclonal antibody which defines and activates cytolytic T lymphocytes. J Immunol 140:1034-42 (1988). PubMed: 3257767

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