APC Anti-Ly6g antibody [RB6-8C5] (ab25273)

Reviews (6)Q&A (3)References (1)

Key features and details

  • APC Rat monoclonal [RB6-8C5] to Ly6g
  • Reacts with: Mouse
  • Conjugation: APC. Ex: 645nm, Em: 660nm
  • Isotype: IgG2b

Overview

  • Product name

    APC Anti-Ly6g antibody [RB6-8C5]
    See all Ly6g primary antibodies

  • Description

    APC Rat monoclonal [RB6-8C5] to Ly6g

  • Host species

    Rat

  • Conjugation

    APC. Ex: 645nm, Em: 660nm

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Target

  • Relevance

    Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.

  • Cellular localization

    Attached to the membrane by a GPI-anchor.

  • Database links

    • Alternative names

      • Gr-1 antibody
      • Gr1 antibody
      • Ly-6G.1 antibody
      • Ly6G antibody
      • Lymphocyte antigen 6 complex locus G antibody
      • Lymphocyte antigen 6G antibody
      see all

    References (1)

    Publishing research using ab25273? Please let us know so that we can cite the reference in this datasheet.

    ab25273 has been referenced in 1 publication.

    • Lyu C  et al. Normal and fibrotic liver parenchyma respond differently to irreversible electroporation. HPB (Oxford) N/A:N/A (2019). PubMed: 30879992

    Customer reviews and Q&As

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    1-9 of 9 Abreviews or Q&A

    Question

    This will be perfect.  Thank you for your quick reply.  I sincerely appreciate the replacement.

    Read More
    Answer

    Thank you for your message and for providing this further information.

    In order to arrange this, I would appreciate if you could confirm your order number and date of purchase?

    Thank you for your continued cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Question

    ReviewID: xxx AbID: 25273, Anti-Ly6g antibody [RB6-8C5] (Allophycocyanin) Rating: Poor Image: Anti-Ly6g antibody [RB6-8C5] (Allophycocyanin) for Flow Cytometry (Dog) Sample: Species: Dog Type: Cell Specification: from whole blood Preparation: Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1% Application: Application: Fixation: Paraformaldehyde Permeabilization: No Gating Strategy: alive cells Other detail: Dilution: 1/100 Incubation time: 1 hour at 4°C Diluent: PBS/BSA 1% Secondary Antibody: Name: None used Conjugation: Additional Data:

    Read More
    Answer

    Thank you for submitting an Abreview for ab25273. As you may have noticed, your review has now been published on our website.
    Since you obtained poor results using the antibody in an untested species (dog), we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.
    Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:
    - fix with PFA after incubation with the primary antibody - if you haven't done that already.
    - incubate longer with the primary antibody (e.g. 2-3 h at RT or overnight at 4 degree)
    It could also just be the case that the antibody does not work for dog samples due to low homology.
    If you decide to follow these suggestions, please let me know if they are helpful.
    I wish you good luck with your research.

    Read More

    Flow Cytometry abreview for Anti-Ly6g antibody [RB6-8C5] (Allophycocyanin)

     Poor
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Dog Cell (from whole blood)
    Specification
    from whole blood
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    alive cells
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 06 2012

    Flow Cytometry abreview for Anti-Ly6g antibody [RB6-8C5] (Allophycocyanin)

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Cynomolgus Monkey Cell (cells from whole blood)
    Specification
    cells from whole blood
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    alive neutrophils
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 02 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Ly6g antibody [RB6-8C5] (Allophycocyanin)

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Spleen from immune mouse)
    Specification
    Spleen from immune mouse
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 16 2012

    Flow Cytometry abreview for Anti-Ly6g antibody [RB6-8C5] (Allophycocyanin)

     Poor
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Rat Cell (Splenocytes)
    Specification
    Splenocytes
    Preparation
    Cell harvesting/tissue preparation method: spleen was mashed
    Sample buffer: PBS/BSA 1%/Azide 0.01%
    Permeabilization
    No
    Gating Strategy
    alive macrophages
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 16 2012

    Question

    I have used a very similar staining protocol to the one you have sent. I would very much appreciate a replacement antibody. Do you have an anti-mouse Gr-1 APC conjugated antibody available? If so, I would prefer to have this be the replacement antibody. Thank you for your support and assistance.

    Read More
    Answer

    Thank you for contacting Abcam. I am sorry to hear you have experienced difficulty obtaining satisfactory results from this antibody.

    I would be happy to arrange a Free of Charge Replacement of this product for you. We do have one product which may suit your needs, our anti-Gr1 antibody AB25273 (https://www.abcam.com/Ly6g-antibody-RB6-8C5-Allophycocyanin-ab25273.html). If this product is suitable for you I will arrange for this to sent to you as soon as possible.

    Thank you for your time and cooperation. We look forward to your reply.

    Read More

    Flow Cytometry abreview for Anti-Ly6g antibody [RB6-8C5] (Allophycocyanin)

     Excellent
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (cells from mouse blood)
    Specification
    cells from mouse blood
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Permeabilization
    No
    Gating Strategy
    on neutrophils
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 20 2011

    Flow Cytometry abreview for Anti-Ly6g antibody [RB6-8C5] (Allophycocyanin)

     Excellent
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (cell from buffy coat)
    Specification
    cell from buffy coat
    Preparation
    Cell harvesting/tissue preparation method: none
    Sample buffer: PBS/BSA 1%
    Permeabilization
    No
    Gating Strategy
    on granulocytes
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 20 2011

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