APC Anti-Ly6g antibody [RB6-8C5] (ab25273)
![Flow Cytometry - APC Anti-Ly6g antibody [RB6-8C5] (ab25273)](https://www.abcam.com/ps/products/25/ab25273/Images/ab25273-459746-APC-AntiLy6g-antibody-flowcytometry-bone-marrow-cells.png "Flow Cytometry - APC Anti-Ly6g antibody [RB6-8C5] (ab25273)")
Key features and details
- APC Rat monoclonal [RB6-8C5] to Ly6g
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
-
Product name
APC Anti-Ly6g antibody [RB6-8C5]
See all Ly6g primary antibodies -
Description
APC Rat monoclonal [RB6-8C5] to Ly6g -
Host species
Rat -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Ly6g. Mouse granulocytes
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Stabilising agent. -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
RB6-8C5 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
- redFluor™ 710 Anti-Ly6g antibody [RB6-8C5] (ab242065)
- PE Anti-Ly6g antibody [RB6-8C5] (ab24884)
- FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)
- violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (ab253070)
- Anti-Ly6g + Ly6c antibody [RB6-8C5] (ab25377)
- PE/Cy7® Anti-Ly6g antibody [RB6-8C5] (ab25514)
- Biotin Anti-Ly6g antibody [RB6-8C5] (ab25680)
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (5) |
Use a concentration of 0.25 µg/ml.
|
| Notes |
|---|
|
Flow Cyt
Use a concentration of 0.25 µg/ml. |
Target
-
Relevance
Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells. -
Cellular localization
Attached to the membrane by a GPI-anchor. -
Database links
- Entrez Gene: 546644 Mouse
- SwissProt: P35461 Mouse
-
Alternative names
- Gr-1 antibody
- Gr1 antibody
- Ly-6G.1 antibody
see all
Images
-
Flow Cytometry - APC Anti-Ly6g antibody [RB6-8C5] (ab25273)
Flow Cytometry analysis of C57Bl/6 bone marrow cells labelling Ly6g with ab25273 at 1.125 ug (solid line) compared to a APC Rat IgG2b isotype control (dashed line) at 0.125 ug
Datasheets and documents
-
Datasheet download
References (1)
ab25273 has been referenced in 1 publication.
- Lyu C et al. Normal and fibrotic liver parenchyma respond differently to irreversible electroporation. HPB (Oxford) N/A:N/A (2019). PubMed: 30879992
