Recombinant APC Anti-Mannose Receptor antibody [EPR6828(B)] (ab223961)
![Flow Cytometry - APC Anti-Mannose Receptor antibody [EPR6828(B)] (ab223961)](https://www.abcam.com/ps/products/223/ab223961/Images/ab223961-312701-anti-mannose-receptor-antibody-epr6828b-allophycocyanin-flow-cytometry.jpg "Flow Cytometry - APC Anti-Mannose Receptor antibody [EPR6828(B)] (ab223961)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EPR6828(B)] to Mannose Receptor
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
Overview
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Product name
APC Anti-Mannose Receptor antibody [EPR6828(B)]
See all Mannose Receptor primary antibodies -
Description
APC Rabbit monoclonal [EPR6828(B)] to Mannose Receptor -
Host species
Rabbit -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Mannose Receptor aa 1400 to the C-terminus. The exact sequence is proprietary.
Database link: P22897 -
Positive control
- Flow Cyt: Molt-4 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6828(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)
- Alexa Fluor® 488 Anti-Mannose Receptor antibody [EPR6828(B)] (ab195191)
- Alexa Fluor® 647 Anti-Mannose Receptor antibody [EPR6828(B)] (ab195192)
- HRP Anti-Mannose Receptor antibody [EPR6828(B)] (ab195193)
- Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)
- Anti-Mannose Receptor antibody [EPR6828(B)] - BSA and Azide free (ab209332)
- PE Anti-Mannose Receptor antibody [EPR6828(B)] (ab223960)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab223961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/50000. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens. -
Sequence similarities
Contains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4360 Human
- Entrez Gene: 17533 Mouse
- Entrez Gene: 291327 Rat
- Omim: 153618 Human
- SwissProt: P22897 Human
- SwissProt: Q61830 Mouse
- Unigene: 75182 Human
- Unigene: 2019 Mouse
see all -
Alternative names
- bA541I19.1 antibody
- C type lectin domain family 13 member D antibody
- C-type lectin domain family 13 member D antibody
see all
Images
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Flow Cytometry - APC Anti-Mannose Receptor antibody [EPR6828(B)] (ab223961)
Overlay histogram showing Molt-4 cells stained with ab223961 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223961, 1/50000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
This antibody gave a positive signal in Molt-4 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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![APC Anti-Mannose Receptor antibody [EPR6828(B)] (ab223961)](https://www.abcam.com/ps/products/223/ab223961/Images/ab223961-3-benefits-of-recombinant-antibodies.png)
APC Anti-Mannose Receptor antibody [EPR6828(B)] (ab223961)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab223961 has not yet been referenced specifically in any publications.
