Recombinant APC Anti-NCAM1 antibody [EPR21827] (ab237383)
![Immunocytochemistry/ Immunofluorescence - APC Anti-NCAM1 antibody [EPR21827] (ab237383)](https://www.abcam.com/ps/products/237/ab237383/Images/ab237383-391582-AntiNCAM1-antibody-EPR21827-Allophycocyanin-immunocytochemistry-neuro2a-mouse.jpg "Immunocytochemistry/ Immunofluorescence - APC Anti-NCAM1 antibody [EPR21827] (ab237383)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EPR21827] to NCAM1
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
Overview
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Product name
APC Anti-NCAM1 antibody [EPR21827]
See all NCAM1 primary antibodies -
Description
APC Rabbit monoclonal [EPR21827] to NCAM1 -
Host species
Rabbit -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment within Mouse NCAM1 aa 1-350. The exact sequence is proprietary.
Database link: P13595 -
Positive control
- Flow Cyt: Neuro2a cells. ICC/IF: Neuro2a cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21827 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab237383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. | |
| ICC/IF | 1/100. |
Target
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Function
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. -
Sequence similarities
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 17967 Mouse
- SwissProt: P13595 Mouse
- Unigene: 439182 Mouse
- Unigene: 4974 Mouse
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Alternative names
- antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
- antigen recognized by monoclonal antibody 5.1H11 antibody
- CD56 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - APC Anti-NCAM1 antibody [EPR21827] (ab237383)
Immunofluorescent analysis of 100% methanol-fixed Neuro2a (Mouse neuroblastoma cell line) cells labeling NCAM1 with ab237383 at 1/100 dilution (5 µg/ml), followed by ab195887 anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 488) (green). The nuclear counterstain is DAPI (blue).
Negative control: L-929 cells.
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![Flow Cytometry - APC Anti-NCAM1 antibody [EPR21827] (ab237383)](https://www.abcam.com/ps/products/237/ab237383/Images/ab237383-391581-AntiNCAM1-antibody-EPR21827-Allophycocyanin-flow-cytometry-neuro2a-mouse.jpg)
Flow Cytometry - APC Anti-NCAM1 antibody [EPR21827] (ab237383)Flow cytometric analysis of L-929 (Mouse connective tissue fibroblast cell line, Left) / Neuro2a (Mouse neuroblastoma cell line, Right) labeling NCAM1 with ab237383 at 1/500 dilution (red) compared with ab232814 Rabbit IgG, monoclonal [EPR25A] - Isotype Control - Allophycocyanin (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).
Negative control: L-929 cells.
Gated on viable cells.
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![APC Anti-NCAM1 antibody [EPR21827] (ab237383)](https://www.abcam.com/ps/products/237/ab237383/Images/ab237383-3-benefits-of-recombinant-antibodies.png)
APC Anti-NCAM1 antibody [EPR21827] (ab237383)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab237383 has not yet been referenced specifically in any publications.
