Recombinant APC Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab267561)
![Flow Cytometry - APC Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab267561)](https://www.abcam.com/ps/products/267/ab267561/Images/ab267561-447582-ab267561RabbitmonoclonalEPR23155317toCD252OX40LAPCHumanHUVECcellsflowcytometry.jpg "Flow Cytometry - APC Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab267561)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EPR23155-317] to OX40L/TNFSF4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
Overview
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Product name
APC Anti-OX40L/TNFSF4 antibody [EPR23155-317]
See all OX40L/TNFSF4 primary antibodies -
Description
APC Rabbit monoclonal [EPR23155-317] to OX40L/TNFSF4 -
Host species
Rabbit -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human OX40L/TNFSF4 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: P23510 -
Positive control
- Flow Cyt: HUVEC cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23155-317 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab267561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/100. |
Target
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Function
Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production. -
Involvement in disease
Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Note=The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T cell-APC interaction and the functional consequences of T cell activation, thereby destabilizing peripheral tolerance. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7292 Human
- Omim: 603594 Human
- SwissProt: P23510 Human
- Unigene: 181097 Human
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Alternative names
- CD 134L antibody
- CD 252 antibody
- CD134 ligand antibody
see all
Images
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Flow Cytometry - APC Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab267561)
Flow cytometry overlay histogram showing positive HUVEC cells (left panel) and negative HeLa cells (right panel) stained with ab267561 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab267561) (1x 106 in 100 µl at 5 µg/ml (1/100)) for 30 mins on ice. Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab267561 has not yet been referenced specifically in any publications.
