Recombinant APC Anti-p75 NGF Receptor antibody [EP1039Y] (ab224996)
![Flow Cytometry - APC Anti-p75 NGF Receptor antibody [EP1039Y] (ab224996)](https://www.abcam.com/ps/products/224/ab224996/Images/ab224996-315862-anti-p75-ngf-receptor-antibody-ep1039y-allophycocyanin-flow-cytometry.jpg "Flow Cytometry - APC Anti-p75 NGF Receptor antibody [EP1039Y] (ab224996)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EP1039Y] to p75 NGF Receptor
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: APC. Ex: 645nm, Em: 660nm
Overview
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Product name
APC Anti-p75 NGF Receptor antibody [EP1039Y]
See all p75 NGF Receptor primary antibodies -
Description
APC Rabbit monoclonal [EP1039Y] to p75 NGF Receptor -
Host species
Rabbit -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human
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Immunogen
Synthetic peptide within Human p75 NGF Receptor aa 350-450. The exact sequence is proprietary.
Database link: P08138 -
Positive control
- Flow Cyt: PC-12 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EP1039Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-p75 NGF Receptor antibody [EP1039Y] (ab195179)
- Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y] (ab195180)
- Anti-p75 NGF Receptor antibody [EP1039Y] - Low endotoxin, Azide free (ab221212)
- Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)
- Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab224996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/5000.
|
Target
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Function
Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. -
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
Domain
Death domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1. -
Post-translational
modificationsN- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4804 Human
- Entrez Gene: 18053 Mouse
- Entrez Gene: 24596 Rat
- Omim: 162010 Human
- SwissProt: P08138 Human
- SwissProt: Q9Z0W1 Mouse
- SwissProt: P07174 Rat
- Unigene: 415768 Human
see all -
Alternative names
- CD271 antibody
- CD271 antigen antibody
- Gp80 LNGFR antibody
see all
Images
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Flow Cytometry - APC Anti-p75 NGF Receptor antibody [EP1039Y] (ab224996)
Overlay histogram showing PC12 cells stained with ab224996 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224996, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
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![APC Anti-p75 NGF Receptor antibody [EP1039Y] (ab224996)](https://www.abcam.com/ps/products/224/ab224996/Images/ab224996-2-benefits-of-recombinant-antibodies.png)
APC Anti-p75 NGF Receptor antibody [EP1039Y] (ab224996)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab224996 has not yet been referenced specifically in any publications.
