Recombinant APC Anti-PDPK1 (phospho S241) antibody [PDK1S241-F7] (ab278756)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [PDK1S241-F7] to PDPK1 (phospho S241)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
Related conjugates and formulations
Overview
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Product name
APC Anti-PDPK1 (phospho S241) antibody [PDK1S241-F7]
See all PDPK1 primary antibodies -
Description
APC Rabbit monoclonal [PDK1S241-F7] to PDPK1 (phospho S241) -
Host species
Rabbit -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PDPK1 (phospho S241). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O15530 -
Positive control
- Flow cyt: HEK-293T cells treated with pervanadate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 99.71% PBS, 0.2% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
PDK1S241-F7 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab278756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 5µl for 106 cells.
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Notes |
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Flow Cyt
Use 5µl for 106 cells. |
Target
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Function
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. -
Tissue specificity
Appears to be expressed ubiquitously. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. -
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 5170 Human
- Omim: 605213 Human
- SwissProt: O15530 Human
- Unigene: 459691 Human
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Alternative names
- 3 phosphoinositide dependent protein kinase 1 antibody
- 3-phosphoinositide-dependent protein kinase 1 antibody
- hPDK 1 antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab278756 has not yet been referenced specifically in any publications.