Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

APC Anti-SIGLEC5 antibody [07] (ab275639)

Submit a review Submit a question
Flow Cytometry - APC Anti-SIGLEC5 antibody [07] (ab275639)

    Key features and details

    • APC Mouse monoclonal [07] to SIGLEC5
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: APC. Ex: 645nm, Em: 660nm
    • Isotype: IgG1

    Overview

    • Product name

      APC Anti-SIGLEC5 antibody [07]
      See all SIGLEC5 primary antibodies

    • Description

      APC Mouse monoclonal [07] to SIGLEC5

    • Host species

      Mouse

    • Conjugation

      APC. Ex: 645nm, Em: 660nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Recombinant fragment corresponding to Human SIGLEC5 aa 1-434. Expressed with six amino acids (LEVLFQ) at the C-terminal was expressed and purified.
      Database link: O15389

    • Positive control

      • Flow Cyt: human whole blood granulocytes.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab275639 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use at an assay dependent concentration.

    Use at 5 μl/Test
    Notes
    Flow Cyt
    Use at an assay dependent concentration.

    Use at 5 μl/Test

    Target

    • Function

      Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

    • Tissue specificity

      Expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.

    • Sequence similarities

      Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Domain

      Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD170 antibody
        • CD170 antigen antibody
        • CD33 antigen-like 2 antibody
        • CD33L2 antibody
        • mSiglec-F antibody
        • OB-binding protein 2 antibody
        • OB-BP2 antibody
        • OBBP2 antibody
        • Obesity-binding protein 2 antibody
        • Sialic acid binding Ig-like lectin 5 antibody
        • Sialic acid binding immunoglobulin-like lectin 5 antibody
        • Sialic acid-binding Ig-like lectin 5 antibody
        • Sialic acid-binding Ig-like lectin F antibody
        • SIGL5_HUMAN antibody
        • Siglec-5 antibody
        • Siglec-F variant antibody
        • Siglec5 antibody
        • Siglecf antibody
        see all

      Images

      • Flow Cytometry - APC Anti-SIGLEC5 antibody [07] (ab275639)
        Flow Cytometry - APC Anti-SIGLEC5 antibody [07] (ab275639)

        Flow cytometric analysis of human whole blood granulocytes labeling SIGLEC5 with ab275639 (Black) compared with isotype control (Grey). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab275639? Please let us know so that we can cite the reference in this datasheet.

      ab275639 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab275639.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com