APC Anti-SIRP alpha antibody [03] (ab275641)
![Flow Cytometry - APC Anti-SIRP alpha antibody [03] (ab275641)](https://www.abcam.com/ps/products/275/ab275641/Images/ab275641-411108-ab2756411AntiFlowCytometryHumanSIRPAAPCU937antibody.JPG "Flow Cytometry - APC Anti-SIRP alpha antibody [03] (ab275641)")
Key features and details
- APC Mouse monoclonal [03] to SIRP alpha
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG2b
Overview
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Product name
APC Anti-SIRP alpha antibody [03]
See all SIRP alpha primary antibodies -
Description
APC Mouse monoclonal [03] to SIRP alpha -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (His-tag) corresponding to Human SIRP alpha aa 1-400 (extracellular). NP_542970.1. Polyhistidine tag at the C-terminus.
Database link: P78324 -
Positive control
- Flow cyt: U937 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
03 -
Isotype
IgG2b -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (1) |
Use at an assay dependent concentration.
10 μl/Test |
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. 10 μl/Test |
Target
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Function
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. -
Tissue specificity
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. -
Sequence similarities
Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 140885 Human
- Omim: 602461 Human
- SwissProt: P78324 Human
- Unigene: 581021 Human
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Alternative names
- Signal regulatory protein alpha type 1 antibody
- Bit antibody
- Brain Ig like molecule with tyrosine based activation motifs antibody
see all
Images
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Flow Cytometry - APC Anti-SIRP alpha antibody [03] (ab275641)
Flow cytometric analysis of U937 (human histiocytic lymphoma cell line) cells labeling SIRP alpha with ab275641 (Black) compared with an isotype control (Grey). Cells should be Fc-blocked by treatment with 20 g of human IgG/106 cells for 1 hour at 4 prior to staining, washed, then stained with ab275641.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab275641 has not yet been referenced specifically in any publications.
