APC Anti-VCAM1 antibody [STA], prediluted (ab103173)
![Flow Cytometry - APC Anti-VCAM1 antibody [STA], prediluted (ab103173)](https://www.abcam.com/ps/products/103/ab103173/Images/ab103173-2-Mouse-monoclonal-VCAM1-sta-APC-prediluted-Human-A549-cells-treated-flow-cytometry.jpg "Flow Cytometry - APC Anti-VCAM1 antibody [STA], prediluted (ab103173)")
Key features and details
- APC Mouse monoclonal [STA] to VCAM1, prediluted
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Overview
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Product name
APC Anti-VCAM1 antibody [STA], prediluted
See all VCAM1 primary antibodies -
Description
APC Mouse monoclonal [STA] to VCAM1, prediluted -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human VCAM1. Human DS6 T cell line
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Positive control
- Flow Cyt: TNF-alpha activated HUVEC cells; Treated A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Conjugates are made from highly purified antibodies (>95% by SDS-PAGE). -
Clonality
Monoclonal -
Clone number
STA -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab103173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 10µl for 106 cells.
or 100µl whole blood
ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
Flow Cyt
Use 10µl for 106 cells. or 100µl whole blood
ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. -
Tissue specificity
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. -
Sequence similarities
Contains 7 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. -
Post-translational
modificationsSialoglycoprotein. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7412 Human
- GenBank: NP_001069.1 Human
- GenBank: NP_001186763.1 Human
- Omim: 192225 Human
- SwissProt: P19320 Human
- Unigene: 109225 Human
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Alternative names
- CD106 antibody
- CD106 Antigen antibody
- INCAM 100 antibody
see all
Images
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Flow Cytometry - APC Anti-VCAM1 antibody [STA], prediluted (ab103173)
Flow cytometry overlay histogram showing wild-type A549 (green line) and VCAM1 knockout A549 cells (red line, ab273758), treated with 10 ng/ml TNF-alpha (ab259410) for 16 h (left) and untreated (right), stained with ab103173. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab103173) (1x106 in 100μl at 0.2μg/ml) for 30 min at 4°C.
Isotype control antibody mouse IgG1κ Allophycocyanin was used at the same concentration and conditions as the primary antibody (wild-type A549 - black line; VCAM knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.
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![Flow Cytometry - APC Anti-VCAM1 antibody [STA], prediluted (ab103173)](https://www.abcam.com/ps/products/103/ab103173/Images/ab103173-319714-anti-vcam1-antibody-sta-prediluted-allophycocyanin-flow-cytometry.jpg)
Flow Cytometry - APC Anti-VCAM1 antibody [STA], prediluted (ab103173)Surface staining of HDLM-2 cells with ab103173.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab103173 has been referenced in 1 publication.
- Leca G et al. Expression of VCAM-1 (CD106) by a subset of TCR gamma delta-bearing lymphocyte clones. Involvement of a metalloprotease in the specific hydrolytic release of the soluble isoform. J Immunol 154:1069-77 (1995). PubMed: 7529789