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    apc-vcam1-antibody-sta-prediluted-ab103173.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cell Adhesion Molecules Vascular
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Validated using a knockout cell line

APC Anti-VCAM1 antibody [STA], prediluted (ab103173)

  • Datasheet
  • SDS
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Flow Cytometry - APC Anti-VCAM1 antibody [STA], prediluted (ab103173)
  • Flow Cytometry - APC Anti-VCAM1 antibody [STA], prediluted (ab103173)

Key features and details

  • APC Mouse monoclonal [STA] to VCAM1, prediluted
  • Suitable for: Flow Cyt
  • Knockout validated
  • Reacts with: Human
  • Conjugation: APC. Ex: 645nm, Em: 660nm
  • Isotype: IgG1

You may also be interested in

Conjugation
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APC Conjugation Kit - Lightning-Link® (ab201807)
Knockout
Product image
Human VCAM1 knockout A549 cell line (ab273758)

View more associated products

Overview

  • Product name

    APC Anti-VCAM1 antibody [STA], prediluted
    See all VCAM1 primary antibodies
  • Description

    APC Mouse monoclonal [STA] to VCAM1, prediluted
  • Host species

    Mouse
  • Conjugation

    APC. Ex: 645nm, Em: 660nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human VCAM1. Human DS6 T cell line

  • Positive control

    • Flow Cyt: TNF-alpha activated HUVEC cells; Treated A549 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    Conjugates are made from highly purified antibodies (>95% by SDS-PAGE).
  • Clonality

    Monoclonal
  • Clone number

    STA
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Vascular
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Endothelial
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell adhesion molecules
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Adhesion molecules ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Atherosclerotic proteins ELISA kits
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • PE Anti-VCAM1 antibody [STA] (ab112074)
    • Biotin Anti-VCAM1 antibody [STA] (ab239243)
  • Isotype control

    • APC Mouse IgG1 [1F8] - Isotype Control (ab91358)
  • KO cell lines

    • Human VCAM1 knockout A549 cell line (ab273758)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab103173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 10µl for 106 cells.

or 100µl whole blood

 

 

 

ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Notes
Flow Cyt
Use 10µl for 106 cells.

or 100µl whole blood

 

 

 

ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • Tissue specificity

    Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • Sequence similarities

    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain

    Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • Post-translational
    modifications

    Sialoglycoprotein.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P19320 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7412 Human
    • GenBank: NP_001069.1 Human
    • GenBank: NP_001186763.1 Human
    • Omim: 192225 Human
    • SwissProt: P19320 Human
    • Unigene: 109225 Human
    • Alternative names

      • CD106 antibody
      • CD106 Antigen antibody
      • INCAM 100 antibody
      • INCAM-100 antibody
      • L1CAM antibody
      • MGC99561 antibody
      • V-CAM 1 antibody
      • Vascular Cell Adhesion Molecule 1 antibody
      • Vascular cell adhesion protein 1 antibody
      • VCAM 1 antibody
      • VCAM-1 antibody
      • VCAM1 antibody
      • VCAM1_HUMAN antibody
      see all

    Images

    • Flow Cytometry - APC Anti-VCAM1 antibody [STA], prediluted (ab103173)
      Flow Cytometry - APC Anti-VCAM1 antibody [STA], prediluted (ab103173)

      Flow cytometry overlay histogram showing wild-type A549 (green line) and VCAM1 knockout A549 cells (red line, ab273758), treated with 10 ng/ml TNF-alpha (ab259410) for 16 h (left) and untreated (right), stained with ab103173. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab103173) (1x106 in 100μl at 0.2μg/ml) for 30 min at 4°C.

      Isotype control antibody mouse IgG1κ Allophycocyanin was used at the same concentration and conditions as the primary antibody (wild-type A549 - black line; VCAM knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

      Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

    • Flow Cytometry - APC Anti-VCAM1 antibody [STA], prediluted (ab103173)
      Flow Cytometry - APC Anti-VCAM1 antibody [STA], prediluted (ab103173)

      Surface staining of HDLM-2 cells with ab103173.

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab103173? Please let us know so that we can cite the reference in this datasheet.

    ab103173 has been referenced in 1 publication.

    • Leca G  et al. Expression of VCAM-1 (CD106) by a subset of TCR gamma delta-bearing lymphocyte clones. Involvement of a metalloprotease in the specific hydrolytic release of the soluble isoform. J Immunol 154:1069-77 (1995). PubMed: 7529789

    Customer reviews and Q&As

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