Overview

  • Product name

  • Description

    Rabbit polyclonal to Apc1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic 17 amino acid peptide from near the C terminal of Human Apc1.

  • Positive control

    • Human testis tissue; SK-N-SH cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 216 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the APC1 family.
    Contains 4 PC repeats.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANAPC 1 antibody
    • Anapc1 antibody
    • Anaphase promoting complex subunit 1 antibody
    • anaphase-promoting complex 1 (meiotic checkpoint regulator) antibody
    • Anaphase-promoting complex subunit 1 antibody
    • Apc 1 antibody
    • APC1 antibody
    • APC1_HUMAN antibody
    • Cyclosome subunit 1 antibody
    • MCPR antibody
    • Meiotic checkpoint regulator antibody
    • Mitotic checkpoint regulator antibody
    • Protein Tsg 24 antibody
    • Protein Tsg24 antibody
    • Testis-specific gene 24 protein antibody
    • TSG 24 antibody
    • TSG24 antibody
    see all

Images

  • Lane 1 : Anti-Apc1 antibody (ab133397) at 1 µg/ml
    Lane 2 : Anti-Apc1 antibody (ab133397) at 2 µg/ml

    Lane 1 : SK-N-SH cell lysate
    Lane 2 : SK-N-SH cell lysate

    Predicted band size: 216 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labelling Apc1 with ab133397 at 5 µg/ml, using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab133397 staining Apc1 in Human PBMC cells by Flow Cytometry. Cells were fixed with formaldehyde and premeabilized. The sample was incubated with the primary antibody (1/100) for 16 hours at 4°C. A FITC-conjugated goat anti-rabbit IgG (1/100) was used as the secondary antibody.

    Gating Strategy: FSC-A/SSC-A.

    See Abreview

References

This product has been referenced in:

  • Lai XL  et al. Apc gene suppresses intracranial aneurysm formation and rupture through inhibiting the NF-?B signaling pathway mediated inflammatory response. Biosci Rep 39:N/A (2019). Read more (PubMed: 30808715) »
  • Sako K  et al. Emi2 mediates meiotic MII arrest by competitively inhibiting the binding of Ube2S to the APC/C. Nat Commun 5:3667 (2014). Read more (PubMed: 24770399) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Flow Cytometry
Fixation
Formaldehyde
Permeabilization
Yes - BD Perm/Wash Buffer
Sample
Human Cell (PBMC)
Specification
PBMC
Gating Strategy
FSC-A/SSC-A
Preparation
Cell harvesting/tissue preparation method: Ficoll isolation of PBMC from whole blood, spin down, wash in FACS buffer 1x, fix, wash 2x, and stain with primary antibody overnight.
Sample buffer: Buffer composition at analysis was 0.1% sodium azide with FBS in phosphate buffered solution.

Abcam user community

Verified customer

Submitted Jul 04 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up