Key features and details
- Rabbit polyclonal to Apc1
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Apc1 antibody
See all Apc1 primary antibodies
DescriptionRabbit polyclonal to Apc1
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic 17 amino acid peptide from near the C terminal of Human Apc1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab133397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 216 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the APC1 family.
Contains 4 PC repeats.
modificationsPhosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis.
- Information by UniProt
- ANAPC 1 antibody
- Anapc1 antibody
- Anaphase promoting complex subunit 1 antibody
Lane 1 : Anti-Apc1 antibody (ab133397) at 1 µg/ml
Lane 2 : Anti-Apc1 antibody (ab133397) at 2 µg/ml
Lane 1 : SK-N-SH cell lysate
Lane 2 : SK-N-SH cell lysate
Predicted band size: 216 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labelling Apc1 with ab133397 at 5 µg/ml, using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab133397 has been referenced in 2 publications.
- Lai XL et al. Apc gene suppresses intracranial aneurysm formation and rupture through inhibiting the NF-?B signaling pathway mediated inflammatory response. Biosci Rep 39:N/A (2019). PubMed: 30808715
- Sako K et al. Emi2 mediates meiotic MII arrest by competitively inhibiting the binding of Ube2S to the APC/C. Nat Commun 5:3667 (2014). PubMed: 24770399