• Product name
    Anti-Apc1 (phospho S355) antibody
    See all Apc1 primary antibodies
  • Description
    Rabbit polyclonal to Apc1 (phospho S355)
  • Host species
  • Specificity
    Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is expected to be phospho-specific for S355 of APC1.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide: SRAHSpPALG conjugated to KLH, corresponding to amino acids 351-359 of Human Apc1.

  • General notes

    This is one of a number of novel phosphorylation sites for APC1 identified in Kraft C et al. It may be involved in the regulation of the APC/C.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This affinity purified antibody is directed against the phosphorylated form of human APC1 at the pS355 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
  • Primary antibody notes
    This is one of a number of novel phosphorylation sites for APC1 identified in Kraft C et al. It may be involved in the regulation of the APC/C.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab10923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa).
ELISA 1/10000 - 1/40000.

Tested in peptide ELISA. Product has been tested against 0.1 µg of the phosphorylated and non-phosphorylated forms of the immunizing peptide.


  • Function
    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the APC1 family.
    Contains 4 PC repeats.
  • Post-translational
    Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANAPC 1 antibody
    • Anapc1 antibody
    • Anaphase promoting complex subunit 1 antibody
    • anaphase-promoting complex 1 (meiotic checkpoint regulator) antibody
    • Anaphase-promoting complex subunit 1 antibody
    • Apc 1 antibody
    • APC1 antibody
    • APC1_HUMAN antibody
    • Cyclosome subunit 1 antibody
    • MCPR antibody
    • Meiotic checkpoint regulator antibody
    • Mitotic checkpoint regulator antibody
    • Protein Tsg 24 antibody
    • Protein Tsg24 antibody
    • Testis-specific gene 24 protein antibody
    • TSG 24 antibody
    • TSG24 antibody
    see all


  • All lanes : Anti-Apc1 (phospho S355) antibody (ab10923) at 1/500 dilution

    Lane 1 : HeLa S3 whole cell lysate (asynchronous cells)
    Lane 2 : HeLa S3 whole cell lysate (cells synchronized at G1/S boundary by treatment with thymidine)
    Lane 3 : HeLa S3 whole cell lysate (cells synchronized at M phase by treatment with nocodazole)

    Lysates/proteins at 30 µg per lane.

    All lanes : Goat anti-Rabbit IgG [H&L] at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 215 kDa
    Observed band size: 215 kDa

    Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division.


This product has been referenced in:
  • Zhang Y  et al. miR-29a suppresses IL-13-induced cell invasion by inhibiting YY1 in the AKT pathway in lung adenocarcinoma A549 cells. Oncol Rep 39:2613-2623 (2018). Read more (PubMed: 29620222) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab10923.
Please use the links above to contact us or submit feedback about this product.


Sign up