Anti-Apc10 antibody (ab106497)
Key features and details
- Rabbit polyclonal to Apc10
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Apc10 antibody
See all Apc10 primary antibodies -
Description
Rabbit polyclonal to Apc10 -
Host species
Rabbit -
Specificity
ab106497 is predicted not to cross-react with other APC family members. Two isoforms of Apc10 are known to exist; ab106497 will recognize both isoforms. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
A 16 amino acid synthetic peptide near the center of Human Apc10 (EAX05052).
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Positive control
- Mouse heart tissue and lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB | (1) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the APC10 family.
Contains 1 DOC domain. - Information by UniProt
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Database links
- Entrez Gene: 68999 Mouse
- SwissProt: Q8K2H6 Mouse
- Unigene: 257445 Mouse
- Unigene: 420790 Mouse
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Form
It localises to Cytosol and nucleoplasm. -
Alternative names
- ANAPC 10 antibody
- anapc10 antibody
- Anaphase promoting complex 10 antibody
see all
Images
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Lane 1 : Anti-Apc10 antibody (ab106497) at 1 µg/ml
Lane 2 : Anti-Apc10 antibody (ab106497) at 2 µg/ml
All lanes : Mouse heart tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 21 kDa -
Immunofluorescence of Apc10 in mouse heart tissue with ab106497 at 20 ug/mL.
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ab106497, at 5 µg/ml, staining Apc10 in Mouse heart tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab106497 has not yet been referenced specifically in any publications.