Key features and details
- Rabbit polyclonal to Apc10
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Apc10 antibody
See all Apc10 primary antibodies
DescriptionRabbit polyclonal to Apc10
Specificityab106497 is predicted not to cross-react with other APC family members. Two isoforms of Apc10 are known to exist; ab106497 will recognize both isoforms.
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
A 16 amino acid synthetic peptide near the center of Human Apc10 (EAX05052).
- Mouse heart tissue and lysate
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months).
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab106497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa.|
|IHC-P||Use at an assay dependent concentration.|
FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the APC10 family.
Contains 1 DOC domain.
- Information by UniProt
FormIt localises to Cytosol and nucleoplasm.
- ANAPC 10 antibody
- anapc10 antibody
- Anaphase promoting complex 10 antibody
Lane 1 : Anti-Apc10 antibody (ab106497) at 1 µg/ml
Lane 2 : Anti-Apc10 antibody (ab106497) at 2 µg/ml
All lanes : Mouse heart tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 21 kDa
Immunofluorescence of Apc10 in mouse heart tissue with ab106497 at 20 ug/mL.
ab106497, at 5 µg/ml, staining Apc10 in Mouse heart tissue by Immunohistochemistry.
ab106497 has not yet been referenced specifically in any publications.