Product nameAnti-Apc10 antibody
See all Apc10 primary antibodies
DescriptionRabbit polyclonal to Apc10
SpecificityThis antibody detects a band at about 26kD that corresponds to Apc10.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
General notesExpiration date is one (1) year from date of opening.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
Our Abpromise guarantee covers the use of ab3629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa).|
FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the APC10 family.
Contains 1 DOC domain.
- Information by UniProt
FormIt localises to Cytosol and nucleoplasm.
- ANAPC 10 antibody
- anapc10 antibody
- Anaphase promoting complex 10 antibody
All lanes : Anti-Apc10 antibody (ab3629) at 1/500 dilution
Lane 1 : Hela nuclear lysates
Lane 2 : Hela cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Predicted band size: 21 kDa
Exposure time: 4 minutes
Western blot using ab 3629 at 1/500.
Secondary ab: goat anti rabbit HRP ab 6721 1/5000
Exposure time: 4 minute
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: 293
ab3629 has not yet been referenced specifically in any publications.