Overview

  • Product name

  • Description

    Rabbit polyclonal to Apc11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    18 amino acid synthetic peptide from an internal sequence of Human Apc11.

  • Positive control

    • Human kidney and uterus tissues; Mouse 3T3 cell tissue lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 9 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May recruit the E2 ubiquitin-conjugating enzymes to the complex.
  • Tissue specificity

    Expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the RING-box family.
    Contains 1 RING-type zinc finger.
  • Domain

    The RING-type zinc finger domain coordinates an additional third zinc ion.
  • Post-translational
    modifications

    Auto-ubiquitinated.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANAPC 11 antibody
    • ANAPC11 antibody
    • Anaphase promoting complex subunit 11 (yeast APC11 homolog) antibody
    • Anaphase promoting complex subunit 11 antibody
    • Anaphase promoting complex subunit 11 homolog (yeast) antibody
    • Anaphase promoting complex subunit 11 homolog antibody
    • Anaphase-promoting complex subunit 11 antibody
    • Apc 11 antibody
    • Apc 11p antibody
    • APC11 anaphase promoting complex subunit 11 homolog (yeast) antibody
    • APC11 anaphase promoting complex subunit 11 homolog antibody
    • APC11 antibody
    • APC11_HUMAN antibody
    • Apc11p antibody
    • Cyclosome subunit 11 antibody
    • Hepatocellular carcinoma associated RING finger protein antibody
    • Hepatocellular carcinoma-associated RING finger protein antibody
    • HSPC 214 antibody
    • HSPC214 antibody
    • MGC882 antibody
    • Yeast APC 11 homolog antibody
    • Yeast APC11 homolog antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human uterus tissue labelling Apc11 with ab133200 at 5 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human kidney tissue labelling Apc11 with ab133200 at 5 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Lane 1 : Anti-Apc11 antibody (ab133200) at 1 µg/ml
    Lane 2 : Anti-Apc11 antibody (ab133200) at 2 µg/ml

    All lanes : 3T3 cell tissue lysate

    Predicted band size: 9 kDa

References

This product has been referenced in:

  • Drouet Y  et al. Integrated analysis highlights APC11 protein expression as a likely new independent predictive marker for colorectal cancer. Sci Rep 8:7386 (2018). IHC-P ; Human . Read more (PubMed: 29743633) »
See 1 Publication for this product

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