Overview

  • Product name
    Anti-Apc11 antibody
    See all Apc11 primary antibodies
  • Description
    Rabbit polyclonal to Apc11
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein corresponding to Human Apc11.

  • Positive control
    • HeLa, NIH-3T3 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: 0.01% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab44708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/400. Predicted molecular weight: 10 kDa.

Target

  • Function
    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May recruit the E2 ubiquitin-conjugating enzymes to the complex.
  • Tissue specificity
    Expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the RING-box family.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING-type zinc finger domain coordinates an additional third zinc ion.
  • Post-translational
    modifications
    Auto-ubiquitinated.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • ANAPC 11 antibody
      • ANAPC11 antibody
      • Anaphase promoting complex subunit 11 (yeast APC11 homolog) antibody
      • Anaphase promoting complex subunit 11 antibody
      • Anaphase promoting complex subunit 11 homolog (yeast) antibody
      • Anaphase promoting complex subunit 11 homolog antibody
      • Anaphase-promoting complex subunit 11 antibody
      • Apc 11 antibody
      • Apc 11p antibody
      • APC11 anaphase promoting complex subunit 11 homolog (yeast) antibody
      • APC11 anaphase promoting complex subunit 11 homolog antibody
      • APC11 antibody
      • APC11_HUMAN antibody
      • Apc11p antibody
      • Cyclosome subunit 11 antibody
      • Hepatocellular carcinoma associated RING finger protein antibody
      • Hepatocellular carcinoma-associated RING finger protein antibody
      • HSPC 214 antibody
      • HSPC214 antibody
      • MGC882 antibody
      • Yeast APC 11 homolog antibody
      • Yeast APC11 homolog antibody
      see all

    References

    ab44708 has not yet been referenced specifically in any publications.

    Publishing research using ab44708? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    4 reviews or Q&A

    10
    Votes: Highest First

    Dear Keith, Thanks for your reply. I really don¹t think its a problem with the secondary. I have used this secondary for 20 years now and it works really well. I have used it with many other antibodies in all of the buffers that I detailed in my e...

    Read More

    Thank you for contacting us. I have spoken with the lab about this issue.They have provided me with the following information. The conditions used in-house are essentially the same as those in your protocol, however, we are unable to locate anything s...

    Read More

    Hello, Would you provide me with an update on when I can expect this antibody please? The last estimate I had was that it would be shipped around 25th Jan but that I would receive an e-mail when it was shipped. I have not had an e-mail. It would ...

    Read More

    Thank you for contacting us.

    It appears that this product is overdue and is not expected to ship for 1 -2 weeks. I unable to find any further information as to the cause of the delay. I am very sorry for the delay. Please let me know if there...

    Read More

    Hello, Thanks for your reply. I am pleased that you will provide a different antibody FOC. From your website, there don’t appear to be any antibodies that are known to work on endogenous protein. However, one of them does show a western with reco...

    Read More

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cause...

    Read More

    DESCRIPTION OF THE PROBLEM No signal or completely black depending upon buffer used (see primary antibody section) SAMPLE Human cell lysate from U2OS cells and DLD-1 cells. PRIMARY ANTIBODY Primary antibody diluted 1/400 in 5% marvel, 5% BSA or Relia...

    Read More

    Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. Have you tried titterin...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up