Overview

  • Product name

    Anti-APC2 antibody [3A2G2]
    See all APC2 primary antibodies
  • Description

    Mouse monoclonal [3A2G2] to APC2
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human APC2 aa 2041-2181. (Expressed in E.coli).
    Database link: O95996

  • Positive control

    • WB: Human APC2 (aa 2041-2181) recombinant protein; APC2 (aa 2041-2181)-hIgGFc transfected HEK-293 cell lysate. Flow Cyt: HeLa cells. ICC/IF: HeLa cells. IHC-P: Human ovarian cancer and bladder cancer tissues.
  • General notes

     This product was previously labelled as Adenomatous polyposis coli protein 2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    3A2G2
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 244 kDa.
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

Target

  • Function

    Promotes rapid degradation of CTNNB1 and may function as a tumor suppressor. May function in Wnt signaling.
  • Tissue specificity

    Widely expressed (at protein level). Specifically expressed in the CNS.
  • Sequence similarities

    Belongs to the adenomatous polyposis coli (APC) family.
    Contains 6 ARM repeats.
  • Developmental stage

    Expressed in fetal brain.
  • Cellular localization

    Cytoplasm, cytoskeleton. Golgi apparatus. Cytoplasm. Cytoplasm, perinuclear region. Associated with actin filaments (PubMed:11691822). Associated with microtubule network (PubMed:10644998, PubMed:11691822).
  • Information by UniProt
  • Database links

  • Alternative names

    • Adenomatous polyposis coli protein 2 antibody
    • Adenomatous polyposis coli protein-like antibody
    • APC-like antibody
    • apc2 antibody
    • APC2_HUMAN antibody
    • APCL antibody
    see all

Images

  • Anti-APC2 antibody [3A2G2] (ab233753) at 1/500 dilution + Human APC2 (aa 2041-2181) recombinant protein

    Predicted band size: 244 kDa



    Expected MW is 40.9 kDa.

  • All lanes : Anti-APC2 antibody [3A2G2] (ab233753) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : APC2 (aa 2041-2181)-hIgGFc transfected HEK-293 cell lysate

    Predicted band size: 244 kDa

  • HeLa (human prostate adenocarcinoma cell line) cells stained for APC2 (green) using ab233753 at 1/200 dilution in ICC/IF.

    Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labelling APC2 with ab233753 at 1/200 dilution (green) compared with a negative control (red).

  • Paraffin-embedded human ovarian cancer tissue stained for APC2 using ab233753 at 1/200 dilution in immunohistochemical analysis. DAB staining.

  • Paraffin-embedded human bladder cancer tissue stained for APC2 using ab233753 at 1/200 dilution in immunohistochemical analysis. DAB staining.

References

ab233753 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233753.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up