• Product name

  • Description

    Rabbit polyclonal to Apc5
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide from a region between residue 705 and the C terminus (residue 755) of human Apc5 (NP_057321.2)

  • Positive control

    • Whole cell lysate from Hela, 293T and NIH3T3 cells.



Our Abpromise guarantee covers the use of ab72516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IP Use at 2-5 µg/mg of lysate.


  • Function

    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the APC5 family.
    Contains 4 TPR repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2510006G12Rik antibody
    • AA408751 antibody
    • AA536819 antibody
    • AA986414 antibody
    • ANAPC 5 antibody
    • ANAPC5 antibody
    • Anaphase promoting complex subunit 5 antibody
    • Anaphase-promoting complex subunit 5 antibody
    • Apc 5 antibody
    • APC5 antibody
    • APC5_HUMAN antibody
    • Cyclosome subunit 5 antibody
    • PDL 108 antibody
    • PDL108 antibody
    • Periodontal ligament fibroblast protein antibody
    • RMC1 antibody
    see all


  • All lanes : Anti-Apc5 antibody (ab72516) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 85 kDa
    Observed band size: 85 kDa

    Exposure time: 3 minutes
  • Immunoprecipitation/ Western Blot of Apc5.
    Lane 1: ab72516 at 3µg/mg Hela whole cell lysate (1mg for IP, 20% of IP loaded).
    Lane 2: Control IgG.
    ab72516 at 1µg/ml for WB.
    Chemiluminescence with an exposure time of 1 minute.


This product has been referenced in:

  • Dai L  et al. Dose-dependent proteomic analysis of glioblastoma cancer stem cells upon treatment with ?-secretase inhibitor. Proteomics : (2011). WB ; Human . Read more (PubMed: 21932445) »
See 1 Publication for this product

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