Key features and details
- Rabbit polyclonal to Apc5
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Apc5 antibody
See all Apc5 primary antibodies
DescriptionRabbit polyclonal to Apc5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide from a region between residue 705 and the C terminus (residue 755) of human Apc5 (NP_057321.2)
- Whole cell lysate from Hela, 293T and NIH3T3 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the APC5 family.
Contains 4 TPR repeats.
- Information by UniProt
- 2510006G12Rik antibody
- AA408751 antibody
- AA536819 antibody
All lanes : Anti-Apc5 antibody (ab72516) at 0.4 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 3 minutes
Immunoprecipitation/ Western Blot of Apc5.
Lane 1: ab72516 at 3µg/mg Hela whole cell lysate (1mg for IP, 20% of IP loaded). Lane 2: Control IgG.
ab72516 at 1µg/ml for WB. Chemiluminescence with an exposure time of 1 minute.
ab72516 has been referenced in 1 publication.
- Dai L et al. Dose-dependent proteomic analysis of glioblastoma cancer stem cells upon treatment with ?-secretase inhibitor. Proteomics : (2011). WB ; Human . PubMed: 21932445