Key features and details
- Rabbit polyclonal to Apc6
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Apc6 antibody
See all Apc6 primary antibodies
DescriptionRabbit polyclonal to Apc6
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- IHC-P: Human kidney tissue. WB: NIH-3T3 cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol
Mg2+ and Ca2+ free
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 71 kDa.|
FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the APC6/CDC16 family.
Contains 7 TPR repeats.
modificationsPhosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.
- Information by UniProt
- ANAPC6 antibody
- Anaphase promoting complex subunit 6 antibody
- Anaphase-promoting complex subunit 6 antibody
All lanes : Anti-Apc6 antibody (ab230926) at 1/500 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell extract
Lane 2 : NIH/3T3 cell extract with Immunizing peptide
Developed using the ECL technique.
Predicted band size: 71 kDa
Formalin-fixed, paraffin-embedded human kidney tissue stained for Apc6 using ab230926 at 1/50 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230926 has not yet been referenced specifically in any publications.