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  1. Link

    apc6-antibody-c-terminal-ab228007.pdf

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Cell Biology Cell Cycle Cell Division Spindle
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Anti-Apc6 antibody - C-terminal (ab228007)

  • Datasheet
  • SDS
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  • Western blot - Anti-Apc6 antibody - C-terminal (ab228007)

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-Apc6 antibody - C-terminal
    See all Apc6 primary antibodies
  • Description

    Rabbit polyclonal to Apc6 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Apc6 (C terminal). The exact sequence is proprietary.
    Database link: Q13042

  • Positive control

    • WB: A431, NCI-H1299 and HeLa S3 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • APC/C

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A431 whole cell lysate (ab30132)
    • A431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human Apc6 protein (ab160243)
  • Related Products

    • Recombinant Human Apc6 protein (ab160243)

Applications

Our Abpromise guarantee covers the use of ab228007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 72 kDa.

Target

  • Function

    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the APC6/CDC16 family.
    Contains 7 TPR repeats.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.
  • Target information above from: UniProt accession Q13042 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 614342 Cow
    • Entrez Gene: 8881 Human
    • Entrez Gene: 69957 Mouse
    • Entrez Gene: 100621739 Pig
    • Entrez Gene: 290875 Rat
    • Entrez Gene: 699089 Rhesus monkey
    • Omim: 603461 Human
    • SwissProt: Q13042 Human
    • SwissProt: Q8R349 Mouse
    • Unigene: 374127 Human
    • Unigene: 182412 Mouse
    • Unigene: 66098 Rat
    see all
  • Alternative names

    • ANAPC6 antibody
    • Anaphase promoting complex subunit 6 antibody
    • Anaphase-promoting complex subunit 6 antibody
    • Apc 6 antibody
    • APC6 antibody
    • CDC 16 antibody
    • CDC16 (cell division cycle 16 S. cerevisiae homolog) antibody
    • Cdc16 antibody
    • CDC16 homolog antibody
    • CDC16 protein antibody
    • CDC16_HUMAN antibody
    • CDC16Hs antibody
    • Cell division cycle 16 antibody
    • Cell division cycle 16 homolog antibody
    • Cell division cycle protein 16 homolog antibody
    • CUT9 antibody
    • Cyclosome subunit 6 antibody
    see all

Images

  • Western blot - Anti-Apc6 antibody - C-terminal (ab228007)
    Western blot - Anti-Apc6 antibody - C-terminal (ab228007)
    All lanes : Anti-Apc6 antibody - C-terminal (ab228007) at 1/500 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
    Lane 3 : HeLa S3 (human lung carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 72 kDa



    7.5% SDS-PAGE

Datasheets and documents

    • Datasheet
    • SDS
  • References

    ab228007 has not yet been referenced specifically in any publications.

    Publishing research using ab228007? Please let us know so that we can cite the reference in this datasheet.

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