Anti-Apc6 antibody - C-terminal (ab228007)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Apc6 antibody - C-terminal
See all Apc6 primary antibodies -
Description
Rabbit polyclonal to Apc6 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Rhesus monkey
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Immunogen
Recombinant fragment within Human Apc6 (C terminal). The exact sequence is proprietary.
Database link: Q13042 -
Positive control
- WB: A431, NCI-H1299 and HeLa S3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab228007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 72 kDa. |
Target
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Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the APC6/CDC16 family.
Contains 7 TPR repeats. -
Post-translational
modificationsPhosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle. - Information by UniProt
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Database links
- Entrez Gene: 614342 Cow
- Entrez Gene: 8881 Human
- Entrez Gene: 69957 Mouse
- Entrez Gene: 100621739 Pig
- Entrez Gene: 290875 Rat
- Entrez Gene: 699089 Rhesus monkey
- Omim: 603461 Human
- SwissProt: Q13042 Human
see all -
Alternative names
- ANAPC6 antibody
- Anaphase promoting complex subunit 6 antibody
- Anaphase-promoting complex subunit 6 antibody
see all
Images
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Western blot - Anti-Apc6 antibody - C-terminal (ab228007)All lanes : Anti-Apc6 antibody - C-terminal (ab228007) at 1/500 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HeLa S3 (human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 72 kDa7.5% SDS-PAGE
References
ab228007 has not yet been referenced specifically in any publications.
