Product nameAnti-Apc6 antibody [EPR11167]
See all Apc6 primary antibodies
DescriptionRabbit monoclonal [EPR11167] to Apc6
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide, corresponding to residues in Human Apc6 (UniProt: Q13042).
- HepG2, HT1080, HUVEC and HeLa cell lysates; HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab169537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 66-72 kDa (predicted molecular weight: 71 kDa).|
|ICC/IF||1/100 - 1/250.|
FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the APC6/CDC16 family.
Contains 7 TPR repeats.
modificationsPhosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.
- Information by UniProt
- ANAPC6 antibody
- Anaphase promoting complex subunit 6 antibody
- Anaphase-promoting complex subunit 6 antibody
All lanes : Anti-Apc6 antibody [EPR11167] (ab169537) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HT1080 cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 71 kDa
Immunofluorescent analysis of HeLa cells labeling Apc6 with ab169537 at 1/100 dilution.
ab169537 has not yet been referenced specifically in any publications.