Overview

  • Product name
    Anti-Apc6 antibody [EPR11167]
    See all Apc6 primary antibodies
  • Description
    Rabbit monoclonal [EPR11167] to Apc6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Apc6 (UniProt: Q13042).

  • Positive control
    • HepG2, HT1080, HUVEC and HeLa cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 66-72 kDa (predicted molecular weight: 71 kDa).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the APC6/CDC16 family.
      Contains 7 TPR repeats.
    • Post-translational
      modifications
      Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
    • Cellular localization
      Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANAPC6 antibody
      • Anaphase promoting complex subunit 6 antibody
      • Anaphase-promoting complex subunit 6 antibody
      • Apc 6 antibody
      • APC6 antibody
      • CDC 16 antibody
      • CDC16 (cell division cycle 16 S. cerevisiae homolog) antibody
      • Cdc16 antibody
      • CDC16 homolog antibody
      • CDC16 protein antibody
      • CDC16_HUMAN antibody
      • CDC16Hs antibody
      • Cell division cycle 16 antibody
      • Cell division cycle 16 homolog antibody
      • Cell division cycle protein 16 homolog antibody
      • CUT9 antibody
      • Cyclosome subunit 6 antibody
      see all

    Images

    • All lanes : Anti-Apc6 antibody [EPR11167] (ab169537) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HT1080 cell lysate
      Lane 3 : HUVEC cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 71 kDa

    • Immunofluorescent analysis of HeLa cells labeling Apc6 with ab169537 at 1/100 dilution.

    References

    ab169537 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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