Recombinant Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16889] to Apc6 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Apc6 antibody [EPR16889] - BSA and Azide free
See all Apc6 primary antibodies -
Description
Rabbit monoclonal [EPR16889] to Apc6 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250509 is the carrier-free version of ab181567.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16889 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the APC6/CDC16 family.
Contains 7 TPR repeats. -
Post-translational
modificationsPhosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle. - Information by UniProt
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Database links
- Entrez Gene: 8881 Human
- Entrez Gene: 69957 Mouse
- Entrez Gene: 290875 Rat
- Omim: 603461 Human
- SwissProt: Q13042 Human
- SwissProt: Q8R349 Mouse
- Unigene: 374127 Human
- Unigene: 182412 Mouse
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Alternative names
- ANAPC6 antibody
- Anaphase promoting complex subunit 6 antibody
- Anaphase-promoting complex subunit 6 antibody
see all
Images
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All lanes : Anti-Apc6 antibody [EPR16889] (ab181567) at 1/5000 dilution
Lane 1 : L-929 (Mouse connective tissue fibroblast cells) whole cell lysate at 20 µg
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg
Lane 4 : HT1080 (Human fibrosarcoma cells) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 71 kDaThis data was developed using ab181567, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Apc6 antibody [EPR16889] (ab181567) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Mouse liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 71 kDaThis data was developed using ab181567, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Apc6 antibody [EPR16889] (ab181567) at 1/5000 dilution
Lane 1 : C6 (Rat glial tumor cells) whole cell lysates
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 71 kDaThis data was developed using ab181567, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab181567, the same antibody clone in a different buffer formulation.APC6 Protein was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab181567 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181567 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract. Lane 2: PBS instead of HeLa whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250509 has not yet been referenced specifically in any publications.