Product nameAnti-Apc6 (phospho T580) antibody
See all Apc6 primary antibodies
DescriptionRabbit polyclonal to Apc6 (phospho T580)
Specificityab12282 is specific for the phosphorylated form of APC6 at the T580 residue. MInimal reactivity is expected with the non-phosphorylated form of the protein.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab12282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/3000. Expect bands of approximately 72kDa, 71.5kDa or 65.9 kDa depending on the isoform (predicted molecular weight: 72 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the APC6/CDC16 family.
Contains 7 TPR repeats.
modificationsPhosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.
- Information by UniProt
- ANAPC6 antibody
- Anaphase promoting complex subunit 6 antibody
- Anaphase-promoting complex subunit 6 antibody
All lanes : Anti-Apc6 (phospho T580) antibody (ab12282) at 1/1000 dilution
Lane 1 : Nocodazole treated HeLa whole cell lysate
Lane 2 : Asynchronous HeLa whole cell lysate
Lysates/proteins at 35 µg per lane.
All lanes : Goat anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 72 kDa
The blot was probed overnight at 4°C with 1/1000 dilution of ab12282.
ab12282 has not yet been referenced specifically in any publications.