• Product name

    Anti-Apc7 antibody
  • Description

    Rabbit polyclonal to Apc7
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Apc7 aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab13734)

  • Positive control

    • This antibody gave a positive control in the following lysates: HeLa Nuclear Extract Heart (Mouse) Tissue Spinal Cord (Mouse) Tissue



Our Abpromise guarantee covers the use of ab4171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
ICC/IF Use a concentration of 10 µg/ml.


  • Function

    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the APC7 family.
    Contains 7 TPR repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • anapc7 antibody
    • Anaphase promoting complex subunit 7 antibody
    • Anaphase-promoting complex subunit 7 antibody
    • APC 7 antibody
    • APC7 antibody
    • APC7_HUMAN antibody
    • Cyclosome subunit 7 antibody
    • Prediabetic NOD sera reactive autoantigen antibody
    see all


  • All lanes : Anti-Apc7 antibody (ab4171)

    Lane 1 : HeLa Nuclear Extract
    Lane 2 : HeLa Nuclear Extract with Apc7 peptide (ab13734) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
    Lane 2 : Goat polyclonal to Rabbit IgG - H&L (HRP) (ab6721) at 1/2000 dilution

    Predicted band size: 63 kDa
    Observed band size: 63 kDa
    Additional bands at: 39 kDa, 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 1 minute

    Western blot using ab4171 against Hela Nuclear Extract.

    The expected band size for Apc7 is 63 kd, which corresponds to the band seen in this western. The band is blocked by the immunising peptide.

    Secondary ab: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000)
    Exposure time: 1min

    Lane 1 and 2: 20µg/lane HeLa Nuclear

    Lane 1: ab4171 (1/500)
    Lane 2: ab4171 (1/500) + 1.0µg ab4171 peptide

  • All lanes : Anti-Apc7 antibody (ab4171) at 1 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Spinal Cord (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.

  • ICC/IF image of ab4171 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4171, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Izawa D & Pines J How APC/C-Cdc20 changes its substrate specificity in mitosis. Nat Cell Biol 13:223-33 (2011). WB ; Human . Read more (PubMed: 21336306) »
  • Nilsson J  et al. The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction. Nat Cell Biol 10:1411-20 (2008). Read more (PubMed: 18997788) »
See all 2 Publications for this product

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